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Using a custom\u2010built incubation chamber, we immunofluorescently assayed cell attachment and morphology on shunt catheters with and without blood after 14\u2009days. Samples exposed to blood showed significantly increased cell attachment (average total cell count 392.0\u00a0\u00b1\u2009317.1 vs. control of 94.7\u00a0\u00b1\u200944.5, <jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.0001). Analysis of the glial fibrillary acidic protein expression showed similar trends (854.4\u00a0\u00b1\u2009450.7 vs. control of 174.3\u00a0\u00b1\u2009116.5, <jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.0001). An in vitro model was developed to represent the exposure of astrocytes to blood following an increase in blood\u2013brain barrier permeability. Exposure of astrocytes to blood increases the number of cells and their spread on the shunt.<\/jats:p>","DOI":"10.1002\/aic.17485","type":"journal-article","created":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T01:47:44Z","timestamp":1633398464000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Response of astrocytes to blood exposure due to shunt insertion in vitro"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4448-1670","authenticated-orcid":false,"given":"Mira","family":"Zaranek","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Materials Science Wayne State University  Detroit Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rooshan","family":"Arshad","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science Wayne State University  Detroit Michigan 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