{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:20:21Z","timestamp":1779106821689,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":12332,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1971,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The rate of proliferation of epithelial cells in the descending colon was investigated in four groups of adult mice sacrificed one hour after injection of <jats:sup>3<\/jats:sup>H\u2010thymidine respectively at 04:00, 10:00, 16:00 and 22:00 h of the day. Semithin (1 \u03bc) Epon embedded sections of the colon were radioautographed after staining with periodic acid\u2010Schiff and iron\u2010hematoxylin.<\/jats:p><jats:p>Measurement of the number of mitoses as well as the labeling indices show a diurnal variation in the rate of proliferation of both vacuolated and mucous cells. The peak of mitotic activity in both cell types seems to be around 12:00\u201313:00 h and the nadir around 20:00\u201322:00 h.<\/jats:p><jats:p>In the vacuolated\u2010columnar cell line, the rate of proliferation of the poorly differentiated cells at the base of the crypt is not much influenced by diurnal variation, while that of the more differentiated cells located higher up is. Mucous cells, which are believed to originate in the poorly differentiated vacuolated cells in the lower portion of the crypt, are also strongly affected. Therefore, the more differentiated cells are, the more susceptible they are to diurnal variation in mitotic activity.<\/jats:p><jats:p>The number of epithelial cells lining the crypt varies throughout the day in a similar manner as the rate of cell proliferation. A slight daily variation is also noted in the number of cells in the surface epithelium. These variations are accounted for by a delayed effect of the population pressure resulting from the mitotic peak. The numbers of vacuolated\u2010columnar and mucous cells vary like those of the whole epithelial population, while argentaffin cells fluctuate more widely. In any case, the three main cell lines of the descending colon of the mouse are influenced to some extent by diurnal variations.<\/jats:p>","DOI":"10.1002\/aja.1001310107","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T19:28:02Z","timestamp":1109446082000},"page":"111-119","source":"Crossref","is-referenced-by-count":23,"title":["Renewal of the epithelium in the descending colon of the mouse. III. Diurnal variation in the proliferative activity of epithelial cells"],"prefix":"10.1002","volume":"131","author":[{"given":"W. W. L.","family":"Chang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"355","article-title":"Influence of the normal flora on mucosal morphology, and cellular renewal in the ileum. A comparison of germ\u2010free, and conventional mice","volume":"12","author":"Abrams G. 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