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This investigation was carried out using one \u03bcm\u2010thick Epon sections which were radioautographed after staining with the periodic acid\u2010Schiff technique and iron\u2010hematoxylin.<\/jats:p><jats:p>In the normalized crypts with ten equal segments, labeled vacuolated cells at one hour after injection of <jats:sup>3<\/jats:sup>H\u2010thymidine were encountered in the lower four segments and in decreasing numbers in segments 5 through 7. From the percent labeled cells in segments of the crypt, the birth rate and fluxes of cells were computed. Moreover, it was found that a cell in the vacuolated\u2010columnar cell line would undergo three mitotic cycles on the average from its birth at the cryptal base to its extrusion from the surface; of these three cycles, the last one which took place from segment 3 to segment 7 appeared to be a changeover from dividing cells to non\u2010dividing cells, in accordance with the \u201cslow cut\u2010off\u201d model of Cairnie et al. ('65b).<\/jats:p><jats:p>Mucous cells located in segments 1 through 6 of the crypt were capable of incorporating <jats:sup>3<\/jats:sup>H\u2010thymidine and thus capable of undergoing mitosis. However, the rate of turnover of mucous cells based on proliferative rate was found to be much lower than the rate of turnover of mucous cells based on the transit time in the non\u2010dividing segments of the crypt. Since there was a concomitant overproduction of cells in the vacuolated\u2010columnar cell line and some morphological similarity between vacuolated cells and newly formed mucous cells in the lower portion of the crypt, it was concluded that some vacuolated cells would give rise to mucous cells. This putative transformation occurred in the lower four segments of the crypt.<\/jats:p><jats:p>Mucous cells which were formed by transformation would migrate upward along the cryptal wall and accumulate more mucus in the theca; in doing so, they would undergo two divisions, on the average, before they became non\u2010dividing mucous cells.<\/jats:p><jats:p>In ascending the cryptal walls, both vacuolated\u2010columnar cells and mucous cells appeared to migrate at a similar speed; they moved much slower at the base of the crypt and accelerated toward the upper portion of the crypt, but they migrated at a constant speed in the non\u2010dividing segments of the crypt.<\/jats:p>","DOI":"10.1002\/aja.1001440104","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T13:57:59Z","timestamp":1109426279000},"page":"39-56","source":"Crossref","is-referenced-by-count":61,"title":["Renewal of the epithelium in the descending colon of the mouse. IV. Cell population kinetics of vacuolated\u2010columnar and mucous cells"],"prefix":"10.1002","volume":"144","author":[{"given":"W. W. L.","family":"Chang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. J.","family":"Nadler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000300303"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001400210"},{"key":"e_1_2_1_4_1","first-page":"457","article-title":"Lymphoepithelial cytomorphosis \u2014 an important means of replacement of epithelial cells","volume":"127","author":"Andrew W.","year":"1957","journal-title":"Anat. 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