{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:26:23Z","timestamp":1771633583450,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":10474,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1976,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fetal rat pancreas (ages 16 to 22 days postcoitum) and neonatal pancreas (4 days postnatal) were grown in organ culture for four days. The medium consisted of chick embryo extract and rooster serum either with or without the addition of corticosterone (3 \u00d7 10<jats:sup>\u22125<\/jats:sup> M). Acinar cell differentiation was assessed using quantitative light microscopic linear scanning of tissue sections and enzymatic analysis of amylase in the culture media and in the explants. In the younger fetal tissue of 16 and 18 days postcoitum exocrine differentiation continued in vitro. The effect of corticosterone was an enhancement of the degree of in vitro differentiation. Even with corticosterone, however, the degree of differentiation in vitro was less than that observed during a similar period in vivo. In differentiated pancreas (20\u2010 and 22\u2010day fetal and 4\u2010day neonatal) the acinar pancreas degenerated under control conditions and a selective growth of the endocrine pancreas was observed. The addition of corticosterone to the media resulted in a maintenance of the differentiated state of the acini except in 22\u2010day fetal tissue in which the acini were not preserved. The differences between these results and the work of other investigators and the possible in vivo role of adreno corticosteroids in exocrine pancreatic differentiation is discussed.<\/jats:p>","DOI":"10.1002\/aja.1001460203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T13:50:04Z","timestamp":1109425804000},"page":"133-149","source":"Crossref","is-referenced-by-count":6,"title":["Fetal and neonatal rat pancreas in organ culture age\u2010related effects of corticosterone on the acinar cell component"],"prefix":"10.1002","volume":"146","author":[{"given":"Robert C.","family":"McEvoy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orion D.","family":"Hegre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnold","family":"Lazarow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"155","article-title":"Hydrocortisone increased glycogen deposition and its dependence on tissue interaction in mouse embryonic lung development in vitro","volume":"27","author":"Alescid T.","year":"1972","journal-title":"J. Embry. Exp. Morphol."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/10.3.329"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001010304"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(72)90069-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"4522","DOI":"10.1016\/S0021-9258(19)42450-2","article-title":"Induction of chymotrypsanogen by hydrocortisone in embryonic chick pancreas in vitro","volume":"249","author":"Cohen A.","year":"1974","journal-title":"J. Biol. Chem."},{"issue":"1","key":"e_1_2_1_7_1","first-page":"200","article-title":"Development of secretory response in pancreatic exocrine cells","volume":"67","author":"Doyle C. M.","year":"1975","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_8_1","first-page":"366","article-title":"Organ culture of fetal rat pancreas: Quantitative analysis by linear scanning of islet and other tissue components","volume":"7","author":"Hegre O. D.","year":"1972","journal-title":"In Vitro"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.22.8.577"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.19.12.906"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-131-34118"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1080339"},{"key":"e_1_2_1_13_1","first-page":"655","article-title":"Researches sur le controle endocrinien de l'accumulation de glycogen dans le foie chez le foetus de rat","volume":"51","author":"Jacquot R.","year":"1959","journal-title":"J. Physiologie"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345690480032701"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-54-6-627"},{"key":"e_1_2_1_16_1","first-page":"307","volume-title":"Perspectives in Biology","author":"Jost A.","year":"1962"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.20.3.399"},{"key":"e_1_2_1_18_1","first-page":"828","article-title":"Evolution de la teneur en corticosterone de la surrenale foetal rat","volume":"158","author":"Kamoun A.","year":"1964","journal-title":"C. R. Soc. Biol. (Paris)"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-015844-0.50015-8"},{"issue":"1","key":"e_1_2_1_20_1","first-page":"169","article-title":"Islet cell transplantation","volume":"6","author":"Leonard R. J.","year":"1974","journal-title":"Kidney International"},{"key":"e_1_2_1_21_1","unstructured":"McEvoy R. C. O. D.HegreandA.Lazarow1973bEffects of adrenocorticoids on fetal pancreatic acinar cell development in organ culture. Endocrine Society Fifty\u2010sixth meeting Chicago p.435 abstract."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.22.8.584"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1975.tb00846.x"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-73-5-535"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(66)80143-X"},{"key":"e_1_2_1_26_1","first-page":"457","article-title":"Pancreas acinar differentiation. I. Morphologic and enzymatic comparisons of embryonic rat pancreas and pancreatic anlage grown in organ culture","volume":"57","author":"Parsa I.","year":"1969","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(72)90083-8"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(67)90022-X"},{"key":"e_1_2_1_29_1","volume-title":"Functions of the Adrenal Cortex","author":"Peron F. G.","year":"1968"},{"key":"e_1_2_1_30_1","first-page":"1267","article-title":"Endocrinologie\u2014Influence de la cortico surrenale sur le contenu en adranaline et noradrenaline de la surrenale, chez le foetus de rat","volume":"260","author":"Roffi J.","year":"1965","journal-title":"C. R. Acad. Sci."},{"key":"e_1_2_1_31_1","first-page":"114","volume-title":"Epithelial\u2010mesenchymal Interactions","author":"Rutter W. J.","year":"1968"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040720403"},{"key":"e_1_2_1_33_1","doi-asserted-by":"crossref","first-page":"3500","DOI":"10.1016\/S0021-9258(19)42600-8","article-title":"The developmental regulation of amylolytic and proteolytic enzymes in the embryonic rat pancreas","volume":"249","author":"Sanders T. G.","year":"1974","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091510109"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88260-9_5"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1096780"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90303-0"}],"container-title":["American Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Faja.1001460203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/aja.1001460203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T15:58:45Z","timestamp":1698854325000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/aja.1001460203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,6]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1976,6]]}},"alternative-id":["10.1002\/aja.1001460203"],"URL":"https:\/\/doi.org\/10.1002\/aja.1001460203","archive":["Portico"],"relation":{},"ISSN":["0002-9106","1553-0795"],"issn-type":[{"value":"0002-9106","type":"print"},{"value":"1553-0795","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,6]]}}}