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Perfusion could be maintained for at least six hours, as demonstrated by the ultrastructure of the mucosal cells and by the constant incorporation of [<jats:sup>3<\/jats:sup>H]\u2010galactose in the surface mucous cells. Moreover all mucous cell types in tissue fixed after six hours of perfusion showed the same histochemical reactions for glycoproteins as in tissue fixed shortly after decapitation of the animal.<\/jats:p><jats:p>The surface mucous cells of the antrum incorporated 30% less [<jats:sup>3<\/jats:sup>H]\u2010galactose, [<jats:sup>3<\/jats:sup>H]\u2010serine and [<jats:sup>35<\/jats:sup>S]\u2010sulphate than those of the fundus. The amount of radioactivity incorporated per cell did not decrease during a subsequent 2 hour chase.<\/jats:p>","DOI":"10.1002\/aja.1001560302","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T13:33:57Z","timestamp":1109424837000},"page":"301-311","source":"Crossref","is-referenced-by-count":7,"title":["Glycoprotein synthesis in the mucous cells of the vascularly perfused rat stomach. I. Surface mucous cells"],"prefix":"10.1002","volume":"156","author":[{"given":"G. A.","family":"Van Huis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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