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The pancreas exhibited an uptake of serotonin which was saturable, with an uptake constant (Km) of 8.75 \u00d7 10<jats:sup>\u22127<\/jats:sup> M, and a Vmax of 873 pmoles per gram. Specificity was determined by the addition of fluoxetine or norepinephrine to the reaction mixture, both at 10<jats:sup>\u22125<\/jats:sup> M. Fluoxetine significantly reduced the <jats:sup>3<\/jats:sup>H\u20105HT uptake, whereas norepinephrine was without effect. Metergoline (10<jats:sup>\u22126<\/jats:sup> M), a specific 5\u2010HT postsynaptic receptor blocker, similarly had no effect on the serotonin uptake in the pancreas. Radioautography of the fragments following uptake of tritiated serotonin (5 \u00d7 10<jats:sup>\u22128<\/jats:sup> M) revealed silver\u2010 grain aggregates dispersed along blood vessels in the interstitial spaces of the exocrine and endocrine pancreas, areas known to be traversed by nerve fibers. There were no silver\u2010 grain aggregates over the exocrine or islet parenchymal cells. These data support the hypothesis that the pancreas is innervated by serotonergic fibers. Further evidence for this hypothesis was provided by a preliminary study demonstrating the presence of tryptophan hydroxylase in pancreatic homogenates. These serotonergic fibers may be involved in the regulation of pancreatic secretion.<\/jats:p>","DOI":"10.1002\/aja.1001590309","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T03:17:23Z","timestamp":1109474243000},"page":"361-368","source":"Crossref","is-referenced-by-count":18,"title":["Specific uptake of tritiated serotonin in the adult rat pancreas: Evidence for the presence of serotonergic fibers"],"prefix":"10.1002","volume":"159","author":[{"given":"Steven B.","family":"Koevary","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"McEvoy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efrain C.","family":"Azmitia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(73)90036-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-89-1-125"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.7.6967079"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1976.tb10407.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(78)90574-X"},{"key":"e_1_2_1_7_1","first-page":"1","article-title":"The occurrence of biogenic monoamines in mammalian endocrine pancreas","volume":"314","author":"Cegrell L.","year":"1968","journal-title":"Acta. 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