{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T02:53:52Z","timestamp":1698029632275},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":7491,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1984,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Young (40 gm) rats were given a single intravenous injection of colchicine (4.0 mg) or vinblastine (2.0 mg). At 10 min after colchicine and 30 min after vinblastine administration, the rats were injected with <jats:sup>3<\/jats:sup>H\u2010fucose. Control rats received <jats:sup>3<\/jats:sup>H\u2010fucose only. All rats were sacrificed 90 min after <jats:sup>3<\/jats:sup>H\u2010fucose injection and their tissues processed for radioautography.<\/jats:p><jats:p>In thyroid follicular cells of control animals, at this time interval, 57% of the total label was associated with colloid and secretory vesicles in the apical cytoplasm while 27% was localized in the Golgi apparatus and neighboring vesicles. In experimental animals, the proportion of label in colloid and apical vesicles was reduced by more than 69% after colchicine and more than 83% after vinblastine treatment. The proportion of label in the Golgi region, on the other hand, increased by more than 125% after colchicine and more than 179% after vinblastine treatment. Within the Golgi region, the great majority of the label was associated with secretory vesicles which accumulated adjacent to the trans face of the Golgi stacks. It is concluded that the drugs do not interfere with passage of newly synthesized thyroglobulin from the Golgi saccules to nearby secretory vesicles, but do inhibit intracellular migration of these vesicles to the cell apex. In most cells the number of vesicles in the apical cytoplasm diminished, but this was not always the case, suggesting that exocytosis may also be partially inhibited. The loss of microtubules in drug\u2010treated cells suggests that the microtubules may be necessary for intracellular transport of thyroglobulin.<\/jats:p>","DOI":"10.1002\/aja.1001700403","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T02:47:03Z","timestamp":1109472423000},"page":"531-543","source":"Crossref","is-referenced-by-count":11,"title":["Influence of colchicine and vinblastine on the intracellular migration of secretory and membrane glycoproteins: II. Inhibition of secretion of thyroglobulin in rat thyroid follicular cells as visualized by radioautography after <sup>3<\/sup>H\u2010fucose injection"],"prefix":"10.1002","volume":"170","author":[{"given":"G.","family":"Wild","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"15","article-title":"Inhibition of secretion of thyroglobulin by vinblastine and colchicine as visualized by radioautography after 3H\u2010fucose injection","volume":"202","author":"Bennett G.","year":"1982","journal-title":"Anat. 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