{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:20:54Z","timestamp":1757701254666},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":7307,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1985,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteopetrosis, a metabolic bone disease characterized by a generalized sclerosis of the skeleton, is inherited as an autosomal recessive in a number of mammalian species. The pathogenesis of congenital osteopetrosis is mediated by a reduction in bone resorption as a result of decreased osteoclast function. This hypothesis is based on both functional and structural evidence of reduced bone resorption in all mutations examined to date. The present study examined the histology of cartilage and bone, the ultrastructure of osteoclasts, and the morphology of mineralized bone surfaces in a lethal osteopetrotic mutation, the osteosclerotic (<jats:italic>oc<\/jats:italic>) mouse.<\/jats:p><jats:p>Histologically, epiphyseal cartilage growth plates, especially the hypertrophic zone, are markedly thickened in <jats:italic>oc<\/jats:italic> mice and metaphyses contain excessive osteoid, features characteristic of rickets. Transmission electron microscopy revealed that less than one\u2010quarter of osteoclasts in <jats:italic>oc<\/jats:italic> mice demonstrated evidence of ruffled border formation compared with three\u2010quarters of the osteoclasts in normal littermates. In mutants, ruffled borders were less elaborate and cytoplasmic processes penetrated into bone surfaces, suggesting that bone may be removed by mechanical rather than by enzymatic means. There was little morphological evidence of cartilage degradation and broad <jats:italic>laminae limitantes<\/jats:italic> persisted in mutants. Mineralized surfaces that undergo resorption in normal mice showed no evidence of bone resorption by scanning EM in mutants.<\/jats:p><jats:p>The presence of a rachitic condition, the observations of reduced bone resorption, and the possible contribution of undermineralized matrices to decreased bone resorption are charcteristics of the osteosclerotic mutation which suggest that it is a unqiue csteopetrotic mutant in which to study both the development and regulation of skeletal metabolism.<\/jats:p>","DOI":"10.1002\/aja.1001720204","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:48:28Z","timestamp":1109440108000},"page":"141-153","source":"Crossref","is-referenced-by-count":37,"title":["Morphological evidence of reduced bone resorption in the osteosclerotic (<i>oc<\/i>) mouse"],"prefix":"10.1002","volume":"172","author":[{"given":"Mark F.","family":"Seifert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Sandy C.","family":"Marks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00432412"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","unstructured":"Boskey A. andS. C.Marks Jr.1984Mineral and matrix alterations in the bones of incisors\u2010absent (ia\/ia) osteopetrotic rats.Calcif. Tissue Int. (in press).","DOI":"10.1007\/BF02554876"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00338800"},{"key":"e_1_2_1_5_1","first-page":"39","volume":"36","author":"Dickie M. 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C.","year":"1984","journal-title":"Clin. Orthop. Relat. Res."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001260305"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-119204-4.50013-2"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","unstructured":"Marks S. C.Jr. M. F.Seifert andP. W.Lane1985Osteosclerosis a recessive skeletal mutation on chromosome 19 in the mouse.J. Hered.(in press).","DOI":"10.1093\/oxfordjournals.jhered.a110059"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198264060-00016"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0221-8747(81)90026-6"},{"key":"e_1_2_1_21_1","first-page":"211","article-title":"Osteopetrosis with rickets in infancy","volume":"23","author":"Ozdirim E.","year":"1981","journal-title":"Turkish J. 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