{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T18:26:44Z","timestamp":1698344804152},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":7279,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1985,3]]},"abstract":"Abstract<\/jats:title>The pit<\/jats:italic> (foveola) of typical mucous units of the pyloric antrum was investigated in 3\u2010 to 4\u2010month\u2010old CD1<\/jats:sub> mice, using light and electron microscopy, sometimes combined with 3<\/jats:sup>H\u2010thuymidine radioautography.<\/jats:p>Reconstruction of units from serial sections revealed that, on the average, the pit measured 151 \u03bcm in length and was lined by 184 mucus\u2010containing pit cells. Of these, 164 were located along the wall of the pit, whereas 20 surrounded its opening on the free surface. For ultrastructural examination the pit was divided into equal thirds. The proximal third, located next to the isthmus and referred to as pit base, was composed of cells showing electrondense mucous granules greater in number but similar in density and diameter to those of isthmal dense granule cells. Nucleoli were rather large, irregular, and reticulated; these and other features were indicative of partial differentiation. The appearance of the cells gradually changed with the distance from the isthmus. In the middle third or mid pit, cells had small, fairly rounded nucleoli, while mucous granules were more numerous than in the pit base but similar in appearance and size; these cells were considered to be mature. In the distal third or pit top\u2010surface, granules became elongated and nucleoli shrank, and lysosomes and vacuoles greatly increased in number, indicating that cells were at a terminal stage. Indeed, some of the cells were extruded into the stomach lumen while others were phagocytosed by adjacent cells. Following a single injection of 3<\/jats:sup>H\u2010thymidine, labeling was found only in a small cohort of cells in the pit base. At the end of 1 day of continuous infusion, the cohort of labeled cells had reached the mid pit; by 2 days, the pit top; and by 3 days, the free surface, where cells were eventually lost. The renewal time of pit cells was assessed at 2.98 days (t1\/2 = 1.8 days), giving a turnover rate of 33.5% per day.<\/jats:p>It is estimated that the divisions of pit base cells provide two\u2010thirds of the cells needed daily for pit\u2010cell renewal, while the other third is supplied by an influx of dense granule cells from the isthmus. These cells enter the pit and continuously migrate toward the gastric lumen, while differentiating in the pit base, maturing in the mid pit, and reaching a terminal stage at the pit topsurface. The progressive and orderly migration of pit cells is described as a \u201cpipeline pattern\u201d of renewal. It is completed in about 3 days when terminal cells are lost at the pit top\u2010surface.<\/jats:p>","DOI":"10.1002\/aja.1001720305","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:48:34Z","timestamp":1109440114000},"page":"225-240","source":"Crossref","is-referenced-by-count":39,"title":["Dynamic histology of the antral epithelium in the mouse stomach: III. Ultrastructure and renewal of pit cells"],"prefix":"10.1002","volume":"172","author":[{"given":"E. R.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1242\/jcs.56.1.83","article-title":"Changes in the size and structure of the nucleolus of columnar cells during their migration from crypt base to villus top in rat jejunum","volume":"56","author":"Altmann G. G.","year":"1982","journal-title":"J. Cell. Sci."},{"key":"e_1_2_1_3_1","first-page":"198","volume-title":"Special Cytology","author":"Bensley R. R.","year":"1932"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001410406"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001260409"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001410403"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/S0016-5085(77)80200-X","article-title":"Cell loss from the normal and stressed gastric mucosae of the rat. A morphological analysis","volume":"72","author":"Harding R. K.","year":"1977","journal-title":"Gastroenterology"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00220822"},{"key":"e_1_2_1_9_1","first-page":"119","article-title":"Classification of cell populations on the basis of their proliferative behavior","volume":"14","author":"Leblond C. P.","year":"1964","journal-title":"Natl. Cancer Inst. Monogr."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001600202"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1152\/physrev.1956.36.2.255","article-title":"Renewal of cell populations","volume":"36","author":"Leblond C. P.","year":"1956","journal-title":"Physiol. Rev."},{"key":"e_1_2_1_12_1","first-page":"460","article-title":"Renewal of the cells in the pyloric epithelium of the adult mouse","volume":"184","author":"Lee E. R.","year":"1976","journal-title":"Anat. 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