{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T04:49:22Z","timestamp":1698382162705},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":5999,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1988,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous examination of dividing cells in the isthmus of the mouse pyloric antrum by using semithin (0.5\u2010\u03bcm\u2010thick) Epon sections revealed that the prophasic condensation of chromosomes began early in the DNA\u2010synthesizing (S) stage. In order to examine whether the same observation could be made in other proliferating cell types, the crypt base columnar ceils in mouse duodenum and the hepatocytes of the rat 48 hr after partial hepatectomy were investigated by morphologic and radioautographic techniques.<\/jats:p><jats:p>When crypt base columnar cells were studied in semi\u2010thin Epon sections, the four phases of mitosis showed the characteristic features described by classical cytologists. Moreover, the proportion of cells in prophase and telophase was high. To relate the mitotic phases to the stages of the cell cycle, the \u201cfrequency of labeled mitoses method\u201d provided the duration of the cell cycle, 12.3 hr, and of the S stage, 7.3 hr. From the frequency of the occurrence of mitotic phases, it was estimated that metaphase lasted 0.3 hr and anaphase 0.11 hr, in line with previous estimates. However, the durations of prophase and telophase were long, 5.9 and 1.9 hr, respectively. The whole mitotic process took over 8 hr. From the duration of prophase and cycle stages, it was calculated that 67% of the S stage was occupied by prophasic cells. In fair agreement with this estimate, 68% of the labeled cells 10 min after a <jats:sup>3<\/jats:sup>H\u2010thymidine injection were found to be in prophase.<\/jats:p><jats:p>In regenerating hepatocytes, the morphological features and frequency of prophase and telophase cells were similar to those observed in duodenal crypt cells. While the cycle time was not measured and, therefore, the duration of cycle stages and mitotic phases could not be estimated, it is likely that their duration would be of the same order of magnitude.<\/jats:p><jats:p>In conclusion, the mitotic process in duodenal crypt cells takes over 8 hr. Moreover, the crypt cells, like antral isthmal cells, show features of early prophase soon after they enter the S stage of the cycle.<\/jats:p>","DOI":"10.1002\/aja.1001830103","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T02:32:54Z","timestamp":1109471574000},"page":"45-56","source":"Crossref","is-referenced-by-count":10,"title":["Visualization of chromosome assembly during the S and G2 stages of the cycle and chromosome disassembly during the G1 stage in semithin sections of mouse duodenal crypt cells and other cells"],"prefix":"10.1002","volume":"183","author":[{"given":"M.","family":"El\u2010Alfy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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