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The animals were killed from 10 min to 14.5 months later, and their lenses were processed for electron microscope radioautography. At 10 min after injection, more than 58% of the silver grains were localized to the Golgi apparatus of the lens epithelial cells. By day 1, the heaviest sites of reaction were the plasma membrane (more than 50% of total label), the basal cytoplasm, and the adjacent lens capsule, where a heavy band of reaction was seen. The remainder of the capsule exhibited a lighter diffuse reaction. In the lens fibers, the label was at first localized to clusters of vesicles but then migrated to the plasma membrane and to the region of the capsule adjacent to the basal surface of these fibers. Light microscope radioautographs of the lens capsule at later time intervals revealed that by 1 month after injection the diffuse reaction had disappeared, and only the strongly labeled band remained. By 14.5 months after injection, this band had migrated partially across the lens capsule, but the capsule itself had increased considerably in thickness. On the other hand, the distance between the labeled band and the free edge of the capsule had decreased from that seen at the time of injection.<\/jats:p>","DOI":"10.1002\/aja.1001830304","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T02:33:40Z","timestamp":1109471620000},"page":"212-225","source":"Crossref","is-referenced-by-count":25,"title":["Synthesis of lens capsule and plasma membrane glycoproteins by lens epithelial cells and fibers in the rat"],"prefix":"10.1002","volume":"183","author":[{"given":"A.","family":"Haddad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(75)90040-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(86)90142-X"},{"key":"e_1_2_1_4_1","first-page":"102","volume-title":"Cell Biology of the Secretory Process","author":"Bennett G.","year":"1984"},{"key":"e_1_2_1_5_1","first-page":"90a","article-title":"Migration of 3H\u2010fucose\u2010labeled glycoproteins to the capsule (basement membrane) of the Iens of the rat eye, as visualized by radioautography","volume":"101","author":"Bennett G.","year":"1985","journal-title":"J. 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