{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T12:40:21Z","timestamp":1698064821282},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":5453,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have modified an existing technique in order to perform DNA analysi by flow cytometry (FCM) of corneal epithelium from the mouse, rat, chicken, rabbit, and human. This protocol permitted an investigation of human corneal scrapings from several categories: normal, aphakic bullous keratopathy (ABK), keratoconus (KC), Fuch's dystrophy, edema, epithelial dysplasia, and lipid degeneration. No abnormal characteristic cell\u2010kinetic profile was detected when averaged DNA histograms were compared statsitically between the normal and either ABK, KC, edema, or Fuch's dystrophy groups. Abnormal DNA histograms were recorded for cell samples that were taken (1) from three individuals who had epithelial dysplasia and (2) from one individual diagnosed with lipid degeneration. The former condition was characterized by histograms that had a subpopulation of cells with an aneuploid amont of DNA or had higher than normal percentages of cells in the S and G<jats:sub>2<\/jats:sub> + M phases of the cell cycle. Corneal cells from the patient who had lipid degeneration had an abnormally high percentage of cells in the G<jats:sub>2<\/jats:sub> + M phases of the cell cycle. The availability of accurate DNA flow cytometric analysis of corneal epithelium allows further studies on this issue from both experimental and clinical situations.<\/jats:p>","DOI":"10.1002\/aja.1001870305","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T16:52:00Z","timestamp":1109436720000},"page":"254-260","source":"Crossref","is-referenced-by-count":3,"title":["Flow cytometric DNA analysis of corneal epithelium"],"prefix":"10.1002","volume":"187","author":[{"given":"E. Robert","family":"Burns","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Roberson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael F.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Shock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Pipkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William G.","family":"Hinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne F.","family":"Anson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"293","article-title":"Nonparametric flow cytometry analysis","volume":"27","author":"Bagwell C. B.","year":"1979","journal-title":"Cytometry"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091990406"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.56.2.605"},{"key":"e_1_2_1_5_1","first-page":"61","article-title":"Circadian influence on the wave form of the frequency of labeled mitoses in mouse corneal epithelium","volume":"8","author":"Burns E. R.","year":"1975","journal-title":"Cell Tissue Kinet."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990040208"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060409"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060106"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990090613"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100116"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030503"}],"container-title":["American Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Faja.1001870305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/aja.1001870305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T13:27:24Z","timestamp":1697981244000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/aja.1001870305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,3]]}},"alternative-id":["10.1002\/aja.1001870305"],"URL":"https:\/\/doi.org\/10.1002\/aja.1001870305","archive":["Portico"],"relation":{},"ISSN":["0002-9106","1553-0795"],"issn-type":[{"value":"0002-9106","type":"print"},{"value":"1553-0795","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3]]}}}