{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:44:24Z","timestamp":1791207864931,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":5392,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1990,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>After 20\u2010day\u2010old rats are placed on a vitamin\u2010A\u2010deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells and a small number of spermatogonia and spermatocytes. Retinol administration of VAD rats reinitiates spermatogenesis, but a stage\u2010synchronization of the seminiferous epithelium throughout the testis of these rats is observed. In order to determine which cell type is responsible for this synchronization, the germ cell population has been analyzed in whole mounts of seminiferous tubules dissected from the testes of rats submitted to the following treatments. Twenty\u2010day\u2010old rats received a VAD diet for 10 weeks and then were divided into three groups of six rats. In group 1, all animals were sacrificed immediately; in group 2, the rats were injected once with retinol and sacrificed 3 hr later; in group 3, the rats were injected once with retinol, placed on a retinol\u2010containing diet for 7 days and 3 hr, and then sacrificed. Three rats from each group had one testis injected with <jats:sup>3<\/jats:sup>H\u2010thymidine 3 hr (groups 1 and 2) or 7 days and 3 hr (group 3) before sacrifice. Three normal adult rats (approximately 100 days old) served as controls. Labeled and unlabeled germinal cells were mapped and scored in isolated seminiferous tubules. In group 1, type A<jats:sub>1<\/jats:sub> and type A<jats:sub>0<\/jats:sub> spermatogonia as well as some preleptotene spermatocytes were present; type A<jats:sub>2<\/jats:sub> A<jats:sub>3<\/jats:sub> A<jats:sub>4<\/jats:sub> In, and B spermatogonia were completely eliminated from the testis. Neither type A<jats:sub>1<\/jats:sub> mitotic figures nor <jats:sup>3<\/jats:sup>H\u2010thymidine\u2010labeled\u2010type A<jats:sub>1<\/jats:sub> nuclei were seen. Three hr after retinol injection (group 2), type A<jats:sub>1<\/jats:sub> mitoses, but no labeled type A<jats:sub>1<\/jats:sub> nuclei were observed. At 7 days and 3 hr after retinol administration (group 3), type A<jats:sub>4<\/jats:sub> and In Spermatogonia as well as type A<jats:sub>1<\/jats:sub> spermatogonia were present. A few residual pachytene spermatocytes were found, and some type A<jats:sub>0<\/jats:sub> cells were labeled. These results indicate that VAD caused, in addition to an impairment of spermatogenesis at the preleptotene spermatocyte step, a selective momentary arrest of surviving type A<jats:sub>1<\/jats:sub> spermatogonia at the G<jats:sub>2<\/jats:sub> phase of their cell cycle. Following administration of vitamin A to VAD rats, these type A<jats:sub>1<\/jats:sub> cells reinitiated spermatogenesis synchronously and, after several cycless of proliferation and renewai, reconstituted the seminiferous epithelium in a stage\u2010synchronized manner.<\/jats:p>","DOI":"10.1002\/aja.1001880107","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T02:24:17Z","timestamp":1109471057000},"page":"57-63","source":"Crossref","is-referenced-by-count":41,"title":["Role of spermatogonia in the stage\u2010synchronization of the seminiferous epithelium in vitamin\u2010A\u2010deficient rats"],"prefix":"10.1002","volume":"188","author":[{"given":"N.","family":"Ismail","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Clermont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"135","article-title":"Renewal of type A spermatogonia in adult rats","volume":"16","author":"Bartmanska J.","year":"1983","journal-title":"Cell Tissue Kinet."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001110202"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001220205"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-76-1-80"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001420203"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000930308"},{"key":"e_1_2_1_8_1","first-page":"37","article-title":"Dur\u00e9e du cycle de l'\u00e9pith\u00e9lium s\u00e9minal du rat","volume":"48","author":"Clermont Y.","year":"1959","journal-title":"Arch. Anat. Microsc. Morphol. Exp."},{"key":"e_1_2_1_9_1","first-page":"124","article-title":"Molecular biology of Sertoli cell","volume":"10","author":"Griswold M. D.","year":"1988","journal-title":"Oxford Rev. Reprod. Biol."},{"key":"e_1_2_1_10_1","first-page":"75","article-title":"DNA synthesis: Proliferation and regeneration of the spermatogonia in the rat","volume":"56","author":"Hilscher W.","year":"1967","journal-title":"Arch. Anat. Microsc. Morphol. 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