{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:08:39Z","timestamp":1791180519317,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":5269,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Anat."],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ultrastructural alterations in duced by colchicine were investigated to determine the secretory activities of odontogenic cells during formation of tooth enameloid matrix in skates. Treated skate inner dental epithelial (IDE) cells did not display dilated cisternae of the granular endoplasmic reticulum (GER) nor accumulate Golgi\u2010associated secretory granules at any dose level or time interval examined. This response was markedly different from that observed in teleost IDE cells synthesizing the enameloid collagen matrix. Treated skate IDE cells did show increased accumulations of glycogencontaining vesicles and intercellular glycogen associated with amorphous material, compared to controls. Additionally, the aberrant occurrence of large intracellular glycogen pools and amorphous material suggested that carbohydrate processing was a major function of skate IDE cells.<\/jats:p><jats:p>Treated odontoblasts associated with enameloid matrix formation sometimes showed dilated GER cisternae, but procollagen secretory granules were not obsered. Instead, electron dense material was present within the Golgi cisternae, tubular granules, and large granules. Some elecron\u2010dense material appeared to be shunted to a resorptive pathway via multivesicular bodies in treated odontoblasts. The continuity of tubular granules with the enameloid matrix suggested that they contained precursors of the enameloid matrix, and possibly the periodic, 17.5\u2010nm crossstriated, \u201cgiant\u201d fibers.<\/jats:p><jats:p>Treated odontoblasts associated with predentin collagen matrix deposition showed dilated GER cisternae and accumulations of procollagen secretory granules, features consistent with the function of active collagen synthesis and secretion.<\/jats:p><jats:p>The findings indicate that (1) skate IDE cells do not synthesize enameloid collagen as found in bony fish tooth development; (2) skate IDE cells do process glycogen for secretion into the enameloid matrix; (3) collagen, although present, is not a major constituent of skate enameloid matrix; (4) enameloid \u201cgiant\u201d fibers are unique to elasmobranchs; and (5) odontoblasts synthesize and secrete proteins other than collagen into the enameloid matrix.<\/jats:p>","DOI":"10.1002\/aja.1001890110","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T02:25:50Z","timestamp":1109471150000},"page":"77-91","source":"Crossref","is-referenced-by-count":12,"title":["The effects of colchicine on the ultrastructure of odontogenic cells in the common skate, <i>Raja erinacae<\/i>"],"prefix":"10.1002","volume":"189","author":[{"given":"Kenneth","family":"Prostak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Seifert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziedonis","family":"Skobe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001430402"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092240212"},{"key":"e_1_2_1_4_1","first-page":"442","volume-title":"Tooth Enamel","author":"Clement J.","year":"1984"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90117-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345790580022701"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051700107"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345790580024501"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(70)80074-0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.5357\/koubyou.45.527"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402340203"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(84)90012-8"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6986656"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02561407"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(80)90040-0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1177\/08959374870010020701","article-title":"Histological and biochemical observations of developing enameloid of the sea bream","volume":"2","author":"Kawasaki K.","year":"1987","journal-title":"Adv. Dent. Res."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1979.tb01910.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051840211"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(70)90157-0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(74)90131-9"},{"key":"e_1_2_1_21_1","first-page":"95","article-title":"Development and structure of the teeth in the dog fish Squalus acanthias (L.) and Scyliorhinus caniculus (L.)","volume":"125","author":"Kerr T.","year":"1955","journal-title":"Proc. R. Soc. Lond."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.154.3753.1192"},{"key":"e_1_2_1_23_1","first-page":"205","article-title":"Porcine \u201cenamelins\u201d cannot be distinguished biochemically from porcine serum albumin and its acid breakdown products","volume":"67","author":"Limeback H.","year":"1988","journal-title":"J. Dent. Res."},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/oso\/9780195049770.001.0001","volume-title":"On Biomineralization","author":"Lowenstam H. A.","year":"1989"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02556334"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02557833"},{"key":"e_1_2_1_27_1","first-page":"319","article-title":"Electron microscopic study on the effects of vinblastine on young odontoblasts in rat incisor","volume":"10","author":"Miake Y.","year":"1982","journal-title":"J. Biol. Buccale"},{"key":"e_1_2_1_28_1","first-page":"53","article-title":"Selachian tooth development. III. Ultrastructural features of secretory amelogenesis in Squalus acanthias","volume":"3","author":"Nanci A.","year":"1983","journal-title":"J. Craniofac. Genet. Dev. Biol."},{"key":"e_1_2_1_29_1","first-page":"569","article-title":"Presence of albumin in developing enamel matrix","volume":"68","author":"Nutman C.","year":"1989","journal-title":"J. Dent. Res."},{"key":"e_1_2_1_30_1","first-page":"65","volume-title":"Dental Morphology and Evolution","author":"Poole D. F. G.","year":"1971"},{"key":"e_1_2_1_31_1","first-page":"1119","article-title":"Collagen fibers in immature enameloid of dogfish teeth","volume":"48","author":"Poole D. F. G.","year":"1969","journal-title":"J. Dent. Res."},{"key":"e_1_2_1_32_1","first-page":"525","volume-title":"Tooth Enamel","author":"Prostak K.","year":"1984"},{"key":"e_1_2_1_33_1","first-page":"75","article-title":"The effects of colchicine on the ultrastructure of the dental epithelium and odontoblasts of teleost tooth buds","volume":"5","author":"Prostak K.","year":"1985","journal-title":"J. Craniofac. Genet. Dev. Biol."},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051870204"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(86)90030-0"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001820106"},{"key":"e_1_2_1_37_1","first-page":"188","volume-title":"Tooth Enamel","author":"Prostak K.","year":"1989"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02405019"},{"key":"e_1_2_1_39_1","first-page":"371","article-title":"Immunochemical homology between elasmobranch scale and tooth extracellular matrix proteins in Cephaloscyllium ventriosum","volume":"7","author":"Samuel N.","year":"1987","journal-title":"J. Craniofac. Gen. Dev. Biol."},{"key":"e_1_2_1_40_1","first-page":"31","volume-title":"Development, Structure and Evolution of Teeth","author":"Shellis P. R.","year":"1978"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1974.0005"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1976.0077"},{"key":"e_1_2_1_43_1","first-page":"43","article-title":"Selachian tooth development: II. Immunolocalization of amelogenin polypeptides in epithelium during secretory amelogenesis in Squalus acanthias","volume":"3","author":"Slavkin H.","year":"1983","journal-title":"J. Craniofac. Gen. Dev. Biol."},{"key":"e_1_2_1_44_1","first-page":"367","article-title":"Petrodentine in extant and fossil dipnoan dentition: Microstructure, histogenesis and growth","volume":"107","author":"Smith M.","year":"1983","journal-title":"Proc. Linn. Soc. N.S.W."},{"key":"e_1_2_1_45_1","first-page":"627","article-title":"The pattern of histogenesis and growth of tooth plates in larval stages of extant lungfish","volume":"140","author":"Smith M.","year":"1985","journal-title":"J. Anat."},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2300423"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(70)90109-3"},{"key":"e_1_2_1_48_1","first-page":"1472","article-title":"Ultrastructural changes of secreting rat\u2010incisor ameloblasts following administration of vincristine and vinblastine","volume":"61","author":"Takuma S.","year":"1982","journal-title":"J. Dent. Res."},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43458-8"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1898.0007"}],"container-title":["American Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Faja.1001890110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/aja.1001890110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T20:27:59Z","timestamp":1698006479000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/aja.1001890110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1002\/aja.1001890110"],"URL":"https:\/\/doi.org\/10.1002\/aja.1001890110","archive":["Portico"],"relation":{},"ISSN":["0002-9106","1553-0795"],"issn-type":[{"value":"0002-9106","type":"print"},{"value":"1553-0795","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]}}}