{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:39:26Z","timestamp":1759883966412,"version":"build-2065373602"},"reference-count":55,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["bsapubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J of Botany"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Premise<\/jats:title><jats:p>Floral scent, usually consisting of multiple compounds, is a complex trait, and its role in pollinator attraction has received increasing attention. However, disentangling the effect of individual floral scent compounds is difficult due to the complexity of isolating the effect of single compounds by traditional methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using available quasi\u2010isogenic lines (qILs) that were generated as part of the original mapping of the floral scent volatile\u2010related loci <jats:italic>CNL1<\/jats:italic> (benzaldehyde) and <jats:italic>TPS2<\/jats:italic> (\u03b2\u2010ocimene) in <jats:italic>Capsella<\/jats:italic>, we generated four genotypes that should only differ in these two compounds. Plants of the four genotypes were introduced into a common garden outside the natural range of <jats:italic>C. rubella<\/jats:italic> or <jats:italic>C. grandiflora<\/jats:italic>, with individuals of a self\u2010compatible <jats:italic>C. grandiflora<\/jats:italic> line as pollen donors, whose different genetic background facilitates the detection of outcrossing events. Visitors to flowers of all five genotypes were compared, and the seeds set during the common\u2010garden period were collected for high\u2010throughput amplicon\u2010based sequencing to estimate their outcrossing rates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Benzaldehyde and \u03b2\u2010ocimene emissions were detected in the floral scent of corresponding genotypes. While some pollinator groups showed specific visitation preferences depending on scent compounds, the outcrossing rates in seeds did not vary among the four scent\u2010manipulated genotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The scent\u2010manipulated <jats:italic>Capsella<\/jats:italic> materials constructed using qILs provide a powerful system to study the ecological effects of individual floral scent compounds under largely natural environments. In <jats:italic>Capsella<\/jats:italic>, individual benzaldehyde and \u03b2\u2010ocimene emission may act as attractants for different types of pollinators.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ajb2.16237","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T04:22:02Z","timestamp":1693801322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Testing the effect of individual scent compounds on pollinator attraction in nature using quasi\u2010isogenic <i>Capsella<\/i> lines"],"prefix":"10.1002","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1655-852X","authenticated-orcid":false,"given":"Ying\u2010Ze","family":"Xiong","sequence":"first","affiliation":[{"name":"School of Life Sciences Central China Normal University  Luoyu Avenue 152 430079 Wuhan China"},{"name":"Institute for Biochemistry and Biology University of Potsdam  Karl\u2010Liebknecht\u2010Stra\u00dfe 24\u201025 14476 Potsdam\u2010Golm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Kappel","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Biology University of Potsdam  Karl\u2010Liebknecht\u2010Stra\u00dfe 24\u201025 14476 Potsdam\u2010Golm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Hagemann","sequence":"additional","affiliation":[{"name":"Applied Zoology\/Animal Ecology Freie Universit\u00e4t Berlin  Haderslebener Str. 9 12163 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friederike","family":"Jantzen","sequence":"additional","affiliation":[{"name":"Applied Zoology\/Animal Ecology Freie Universit\u00e4t Berlin  Haderslebener Str. 9 12163 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Wozniak","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Biology University of Potsdam  Karl\u2010Liebknecht\u2010Stra\u00dfe 24\u201025 14476 Potsdam\u2010Golm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"Sicard","sequence":"additional","affiliation":[{"name":"Department of Plant Biology Uppsala Biocenter  BOX 7080 750 07 Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang\u2010Quan","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Central China Normal University  Luoyu Avenue 152 430079 Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8661-6911","authenticated-orcid":false,"given":"Michael","family":"Lenhard","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Biology University of Potsdam  Karl\u2010Liebknecht\u2010Stra\u00dfe 24\u201025 14476 Potsdam\u2010Golm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16035"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14496"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-003-1336-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13639"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15747"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(00)00117-7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1163\/157075606777304221"},{"key":"e_1_2_9_9_1","first-page":"197","volume-title":"Practical pollination biology","author":"Dobson H. 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