{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:34:20Z","timestamp":1761100460736},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,18]],"date-time":"2001-12-18T00:00:00Z","timestamp":1008633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Twenty\u2010two multi\u2010transfused patients with a long duration of severe aplastic anemia (SAA) received a transplant from an HLA\u2010matched donor after cyclophosphamide (CY) plus antithymocyte globulin plus procarbazine using CD34<jats:sup>+<\/jats:sup> enriched blood stem cells + fresh marrow. Peripheral blood stem cells (PBSC) were collected on days 5 and 6 of G\u2010CSF (10 \u03bcg\/kg\/day), and T cells were depleted using an immunoadsorption column (<jats:italic>n<\/jats:italic> = 15) or magnetic cell sorting (<jats:italic>n<\/jats:italic> = 7). Marrow harvesting was performed 48 hr after the last leukapheresis. Two patients (9.1%) that developed graft failure had a successful engraftment again using unpurged PBSC. Median time to neutrophils \u22650.5 \u00d7 10<jats:sup>9<\/jats:sup>\/l and platelets \u226520 \u00d7 10<jats:sup>9<\/jats:sup>\/l without platelet transfusions were 12 days and 17 days, respectively. Acute graft\u2010versus\u2010host disease (GVHD) grade II occurred in four of 22 patients. No patient developed grade III or IV acute GVHD. Four of the evaluable 21 patients had chronic GVHD. One patient developed extensive disease. Three patients (13.6%) died from CY\u2010induced heart failure, extensive\u2010type chronic GVHD, and sepsis of unknown cause. The Kaplan\u2013Meier estimate of survival was 83.9% (95% CI, 70.1\u201395.2%) with a median follow\u2010up duration of 33.5 (6\u201344) months. CD34<jats:sup>+<\/jats:sup>\u2010enriched PBSC in combination with unmanipulated marrow seem to play a role in overcoming the sensitization to histocompatibility antigens without an apparent increase in GVHD. The stem cell component therapy may be feasible for the high\u2010risk SAA adult patients. Am. J. Hematol. 69:15\u201322, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.10015","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T22:17:27Z","timestamp":1030313847000},"page":"15-22","source":"Crossref","is-referenced-by-count":12,"title":["Supplemental peripheral blood stem cells to decrease marrow rejection in adult patients with severe aplastic anemia"],"prefix":"10.1002","volume":"69","author":[{"given":"Chang\u2010Ki","family":"Min","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Wook","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Wook","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi Wha","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo Sung","family":"Min","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Choo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-199803000-00002"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(05)70481-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000041000"},{"key":"e_1_2_1_5_2","first-page":"858","article-title":"Bone marrow transplantation for severe aplastic anemia: has outcome improved?","volume":"90","author":"Passweg JR","year":"1997","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90028-3"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V59.2.236.236"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.1.269.269"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.3.941.941"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1182\/blood.V68.6.1363.1363","article-title":"Decreased incidence of marrow graft rejection in patients with severe aplastic anemia: changing impact of risk factors","volume":"68","author":"Deeg HJ","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1988.tb07597.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701986"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702202"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701585"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1700608"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1182\/blood.V48.1.63.63","article-title":"Severe aplastic anemia; 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