{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:53:24Z","timestamp":1791140004500,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,5,29]],"date-time":"2002-05-29T00:00:00Z","timestamp":1022630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Comit\u00e9 D\u00e9partemental de la Lutte contre le Cancer de la Loire"},{"name":"Association de la Recherche contre le Cancer"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Excessive apoptosis is implicated in the pathogenesis of myelodysplastic syndromes (MDS). We assessed by flow cytometry the expression of several members of the Bcl\u20102 family in bone marrow mononuclear cells (BMMNC) of 168 MDS samples at diagnosis. The proteins studied were Bcl\u20102, Bcl\u2010xL (anti\u2010apoptotic), Bax, Bad, Bak, and Bcl\u2010xS (pro\u2010apoptotic). The percentage of BMMNC expressing Bcl\u20102 and Bcl\u2010xL was higher in refractory anemia with excess of blasts (RAEB), RAEB in transformation (RAEB\u2010T), and chronic myelomonocytic leukemia (CMML) than in refractory anemia (RA) and RA with ringed sideroblasts (RAS). Conversely pro\u2010apoptotic proteins Bad, Bak, and Bcl\u2010xS were detected in a higher percentage of cells in RA and RAS. RA and RAS were associated with an increased Bcl\u2010xS\/Bcl\u2010xL ratio. The expression of anti\u2010apoptotic proteins was also correlated with that of CD34 and P170 and with the percentage of blast cells. Two\u2010color analyses demonstrated that CD34 and Bcl\u20102 were usually expressed in the same cells. No significant correlation was found with cytogenetic abnormalities. Higher expression of pro\u2010apoptotic Bcl\u20102\u2010family proteins (Bak, Bad, Bcl\u2010xS) and higher Bcl\u2010xS\/Bcl\u2010xL ratio were associated with longer survival and decreased risk of leukemic transformation in univariate analysis, whereas expression of anti\u2010apoptotic proteins was associated with decreased survival. Consequently Bcl\u20102 proteins expression was well correlated with the International Prognostic Scoring System (IPSS). Our data confirm that the control of apoptosis is deregulated in MDS cells. Moreover, the study of markers such as CD34 (or Bcl\u20102), Bcl\u2010xL, and Bcl\u2010xS provides additional prognostic information. Am. J. Hematol. 70:115\u2013125, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.10108","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:35:39Z","timestamp":1033893339000},"page":"115-125","source":"Crossref","is-referenced-by-count":53,"title":["Expression and prognostic significance of Bcl\u20102 family proteins in myelodysplastic 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