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We have established a technique to quantify <jats:bold><jats:italic>Aspergillus<\/jats:italic><\/jats:bold> DNA using a real\u2010time PCR method to resolve this problem, and we report herein a successful application of real\u2010time PCR to diagnose invasive pulmonary aspergillosis by comparing the amount of <jats:bold><jats:italic>Aspergillus<\/jats:italic><\/jats:bold> DNA in bronchial lavage fluid from an affected area to that from an unaffected area. This novel tool will provide rapid, sensitive, and specific diagnosis of pulmonary aspergillosis. Am. J. Hematol. 72:27\u201330, 2003. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.10259","type":"journal-article","created":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T02:20:13Z","timestamp":1041387613000},"page":"27-30","source":"Crossref","is-referenced-by-count":31,"title":["Rapid diagnosis of invasive pulmonary aspergillosis by quantitative polymerase chain reaction using bronchial lavage 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