{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:03:03Z","timestamp":1792371783242,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,1,23]],"date-time":"2004-01-23T00:00:00Z","timestamp":1074816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Red blood cells from patients with sickle cell disease (SS RBC) adhere to laminin and over\u2010express the high\u2010affinity laminin receptor basal cell adhesion molecule\/Lutheran protein (B\u2010CAM\/LU). This receptor has recently been shown to undergo activation in vitro through a protein kinase A\u2010dependent mechanism. Low\u2010density SS RBC express two\u2010thirds more B\u2010CAM\/LU than high\u2010density SS RBC. However, high\u2010density SS RBC have been identified as most adherent to laminin under flow conditions. We investigated the ability of low\u2010 and high\u2010density SS RBC to interact with laminin under various conditions and explored factors that might be responsible for the differences in B\u2010CAM\/LU\u2013laminin interaction between high\u2010 and low\u2010density SS RBC. We confirmed that high\u2010density SS RBC adhere to laminin more strongly than low\u2010density SS RBC under flow conditions. However, low\u2010density SS RBC bind soluble laminin most strongly and are the most adherent to laminin under static conditions. Soluble recombinant Lutheran extracellular domain protein completely blocked SS RBC adhesion to laminin under both static and flow conditions. The protein kinase A inhibitor 14\u201022 amide inhibited adhesion to laminin during flow by high\u2010density SS RBC from patients with strongly adherent cells but had no effect on adhesion observed after a static phase. Deletion of the cytoplasmic domain of B\u2010CAM as well as mutation of the juxtamembranous tyrosine residue failed to reduce B\u2010CAM\u2010mediated adhesion to laminin by transfected MEL cells. These studies confirm that B\u2010CAM\/LU is the most critical receptor mediating adhesion to laminin under both static and flow conditions. Dense SS RBC are most adherent to laminin despite bearing fewer laminin receptors, apparently due to a reversible protein kinase A\u2010dependent process that is unlikely to involve direct phosphorylation of B\u2010CAM\/LU. Our results also suggest that the nature of the interaction of B\u2010CAM\/LU with laminin may be different under static and flow conditions. Am. J. Hematol. 75:63\u201372, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.10442","type":"journal-article","created":{"date-parts":[[2004,1,27]],"date-time":"2004-01-27T09:00:29Z","timestamp":1075194029000},"page":"63-72","source":"Crossref","is-referenced-by-count":31,"title":["B\u2010CAM\/LU expression and the role of B\u2010CAM\/LU activation in binding of low\u2010 and high\u2010density red cells to laminin in sickle cell disease"],"prefix":"10.1002","volume":"75","author":[{"given":"Qin","family":"Zen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Batchvarova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Twyman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine E.","family":"Eyler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiling","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura M.","family":"De Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn J.","family":"Telen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,1,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"127","article-title":"Relation of increased hypotonic fragility and of erythrostasis to the mechanism of hemolysis in certain anemias","volume":"55","author":"Ham TH","year":"1940","journal-title":"Trans Assoc Am Phys"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3961486"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112696"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109646"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830050303"},{"key":"e_1_2_1_7_2","first-page":"105","article-title":"Elevated fibrin D\u2010dimer fragment in sickle cell anemia: evidence for activation of coagulation during the steady state as well as in painful crisis","volume":"19","author":"Francis RB","year":"1989","journal-title":"Haemostasis"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V68.1.317.317"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"4879","DOI":"10.1182\/blood.V87.11.4879.bloodjournal87114879","article-title":"Increased adhesion of erythrocytes to components of the extracellular matrix: isolation and characterization of a red blood cell lipid that binds thrombospondin and laminin","volume":"87","author":"Hillery CA","year":"1996","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00159a001"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19960616)61:4<493::AID-JCB2>3.0.CO;2-J"},{"key":"e_1_2_1_12_2","first-page":"611","article-title":"Elevated immunoreactive tumor necrosis factor and interleukin\u20101 in sickle cell disease","volume":"84","author":"Francis RB","year":"1992","journal-title":"J Natl Med Assoc"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.7.2451"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"4845","DOI":"10.1182\/blood.V87.11.4845.bloodjournal87114845","article-title":"Adhesion of sickle red blood cells and damage to interleukin\u20101 beta stimulated endothelial cells under flow in vitro","volume":"87","author":"Natarajan M","year":"1996","journal-title":"Blood"},{"key":"e_1_2_1_15_2","first-page":"450a","article-title":"Relationship of sickle red blood cell laminin binding to disease activity","volume":"100","author":"De Castro LM","year":"2002","journal-title":"Blood"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1204"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1182\/blood.V92.8.2951","article-title":"Sickle cell adhesion to laminin: potential role for the \u03b15 chain","volume":"92","author":"Lee SP","year":"1998","journal-title":"Blood"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.1.312"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5496"},{"key":"e_1_2_1_20_2","first-page":"5761","article-title":"Molecular cloning of the B\u2010CAM cell surface glycoprotein of epithelial cancers: a novel member of the immunoglobulin superfamily","volume":"54","author":"Campbell IG","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.27.16686"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.2.728"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102978200"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10817"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2001-12-0289"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365519709099402"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.3.1179.bloodjournal8731179"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42204-6"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950190208"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(95)80110-3"},{"key":"e_1_2_1_31_2","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1023\/A:1006754829564","article-title":"Role of the cytoskeleton in adhesion stabilization of human colorectal carcinoma cells to extracellular matrix components under dynamic conditions of laminar flow","volume":"17","author":"Haier J","year":"1999","journal-title":"Clin Exp Metastasis"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02757.x"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112144"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830190203"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.256.3.C579"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.3.860"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.4.1293.004k42_1293_1300"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.10442","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.10442","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T16:42:10Z","timestamp":1700239330000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.10442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,23]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1002\/ajh.10442"],"URL":"https:\/\/doi.org\/10.1002\/ajh.10442","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1,23]]}}}