{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T14:10:07Z","timestamp":1700403007475},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,3,22]],"date-time":"2001-03-22T00:00:00Z","timestamp":985219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aggressive multiple myeloma with high serum lactate dehydrogenase (LDH) often has unusual clinical features and is considered to be a distinct clinical entity of multiple myeloma. A myeloma cell line, designated Maska\u201098, was established from the bone marrow of a patient with aggressive myeloma with extremely high serum LDH that was resistant to conventional chemotherapy. Maska\u201098 cells had morphological features of immature plasma cells, and immunophenotypic analysis showed that the cells expressed the plasma cell\u2010associated surface antigens including CD38, 49d, and 56, but no T\u2010 or B\u2010cell antigens, such as CD2, 3, 4, 8, 19, and 20. Maska\u201098 cells contained cytoplasmic immunoglobulin (IgG \u03bb). By utilizing this cell line we demonstrated that the myeloma cells produce and release a large amount of LDH, since (i) abundant LDH was found in the culture supernatant of Maska\u201098, (ii) immunocytochemical analysis showed that cytoplasm of the cells was strongly stained with anti\u2010LDH monoclonal antibody, and (iii) Maska\u201098 cells expressed a greater amount of LDH mRNA than the T\u2010cell line TALL\u20101, as shown by reverse transcription\u2013polymerase chain reaction. As far as we know, there is no report of a myeloma cell line producing excess LDH. Therefore, Maska\u201098 would provide a novel source for further studies of the pathogenesis of aggressive multiple myeloma with high serum LDH. Am. J. Hematol. 66:267\u2013273, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.1056","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:27:27Z","timestamp":1030310847000},"page":"267-273","source":"Crossref","is-referenced-by-count":1,"title":["Lactate dehydrogenase production and release in a newly established human myeloma cell 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