{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:23:44Z","timestamp":1758630224977},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,10,23]],"date-time":"2001-10-23T00:00:00Z","timestamp":1003795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Essential thrombocythemia (ET) is a clonal myeloproliferative disorder characterized by an elevation of platelets in peripheral blood and excessive proliferation of megakaryocytes in bone marrow. The pathological mechanisms for the elevation of megakaryocytes and platelets in ET remain unclear. To study the hypersensitivity of megakaryocyte progenitors to thrombopoietin (TPO), a 9\u2010year\u2010old girl, diagnosed with ET, underwent dose\u2010response experiments with recombinant human TPO, using her bone marrow non\u2010adherent mononuclear cells and CD34 positive cells. Spontaneous colony\u2010forming unit\u2010megakaryocytes (CFU\u2010Meg) were observed in serum\u2010deprived cultures of non\u2010adherent mononuclear cells, whereas they disappeared in cultures of CD34 positive cells. The patient's CFU\u2010Meg showed maximal growth at concentrations of TPO lower than those for normal children. Dose\u2010response curves demonstrated a 50\u201380 fold increase in sensitivity of the patient's CFU\u2010Meg to TPO. We observed hypersensitivity of megakaryocyte progenitors to TPO in a child with ET. Our results suggest that spontaneous CFU\u2010Meg formation in patients with ET may be due to hypersensitivity to TPO. Am. J. Hematol. 68:194\u2013197, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.1178","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T16:51:44Z","timestamp":1030294304000},"page":"194-197","source":"Crossref","is-referenced-by-count":7,"title":["Hypersensitivity of megakaryocyte progenitors to thrombopoietin in essential thrombocythemia"],"prefix":"10.1002","volume":"68","author":[{"given":"Hirohide","family":"Kawasaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahide","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urara","family":"Kohdera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohnosuke","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"29","article-title":"Experience of the Polycythemia vera study group with essential thrombocythemia: a final report on diagnostic criteria, survival, and leukemic transition by treatment","volume":"34","author":"Murphy S","year":"1997","journal-title":"Semin Hematol"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.2.419.bloodjournal862419"},{"key":"e_1_2_1_4_2","first-page":"843","article-title":"Clonal growth of human megakaryocyte progenitors in serum\u2010free cultures: effect of recombinant human interleukin 3","volume":"16","author":"Teramura M","year":"1988","journal-title":"Exp Hematol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1182\/blood.V59.2.447.447","article-title":"Erythroid progenitors in polycythemia vera: demonstration of their hypersensitivity to erythropoietin using serum free cultures","volume":"59","author":"Casadevall N","year":"1982","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115009"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1182\/blood.V80.4.891.891","article-title":"Polythemia vera. 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