{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T02:43:16Z","timestamp":1700188996029},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T00:00:00Z","timestamp":1090540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gene amplification is a relatively rare event in hematologic malignancies. The <jats:italic>ABL<\/jats:italic> gene on chromosome band 9q34 is a proto\u2010oncogene and is the well\u2010known translocation partner of the <jats:italic>BCR<\/jats:italic> gene on 22q11 giving rise to t(9;22)(q34;q11), which is the hallmark of chronic myeloid leukemia and is the most common chromosomal abnormality in adult acute lymphoblastic leukemia (ALL). Amplification of <jats:italic>ABL<\/jats:italic> is an exceedingly rare event, with only less than 5 cases reported in the literature. The <jats:italic>p16<\/jats:italic><jats:sup><jats:italic>INK4a<\/jats:italic><\/jats:sup> (or <jats:italic>CDKN2A<\/jats:italic>) gene on 9p21 is a tumor suppressor gene, and deletion thereof is recently recognized as one of the most common genetic abnormalities in ALL. The authors herein describe an 8\u2010year\u2010old male patient with precursor T\u2010cell ALL harboring both <jats:italic>ABL<\/jats:italic> gene amplification and <jats:italic>p16<\/jats:italic><jats:sup><jats:italic>INK4a<\/jats:italic><\/jats:sup> gene deletion. Fluorescence in situ hybridization (FISH) analysis using <jats:italic>BCR<\/jats:italic>\/<jats:italic>ABL<\/jats:italic> probes revealed five or more <jats:italic>ABL<\/jats:italic> signals, indicating amplification in 51.5% of interphase nuclei. FISH using <jats:italic>p16<\/jats:italic><jats:sup><jats:italic>INK4a<\/jats:italic><\/jats:sup> gene probes showed heterozygous <jats:italic>p16<\/jats:italic><jats:sup><jats:italic>INK4a<\/jats:italic><\/jats:sup> deletion in 71.0%. On conventional cytogenetic analysis, however, only 10 metaphases were available, which showed the normal karyotype, 46,XY[10], serving no evidence for the findings on FISH. This is the first report of an ALL case with <jats:italic>ABL<\/jats:italic> amplification, and the authors speculate that both <jats:italic>ABL<\/jats:italic> proto\u2010oncogene amplification and the <jats:italic>p16<\/jats:italic><jats:sup><jats:italic>INK4a<\/jats:italic><\/jats:sup> tumor suppressor gene deletion have been implicated in leukemogenesis in the present case, although whether the <jats:italic>ABL<\/jats:italic> amplification truly contributes to the leukemogenesis or merely an epiphenomenon representing underlying genomic instability remains to be determined. Am. J. Hematol. 76:360\u2013363, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.20117","type":"journal-article","created":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T17:03:38Z","timestamp":1090602218000},"page":"360-363","source":"Crossref","is-referenced-by-count":14,"title":["<i>ABL<\/i> oncogene amplification with <i>p16<\/i><sup><i>INK4a<\/i><\/sup> gene deletion in precursor T\u2010cell acute lymphoblastic leukemia\/lymphoma: Report of the first case"],"prefix":"10.1002","volume":"76","author":[{"given":"Hee\u2010Jin","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee\u2010Yeon","family":"Woo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Hoe","family":"Koo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun\u2010Young","family":"Tak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun\u2010Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.160.3825.272"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2000.18.21.3651"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.20.3.719"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200104053441401"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402741"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1182\/blood.V85.9.2321.bloodjournal8592321","article-title":"Frequent deletion of p16INK4a\/MTS1 and p15INK4b\/MTS2 in pediatric acute lymphoblastic leukemia","volume":"85","author":"Okuda T","year":"1995","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402697"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0609.2002.00547.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1182\/blood.V89.2.596","article-title":"Frequent jumping translocations of chromosomal segments involving the ABL oncogene alone or in combination with CD3\u2010MLL genes in secondary leukemias","volume":"89","author":"Tanaka K","year":"1997","journal-title":"Blood"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050337"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1999.0126"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870140308"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(97)00119-7"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199910)26:2<166::AID-GCC9>3.0.CO;2-P"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03937.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.1188"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11090"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.20117","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.20117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T02:16:47Z","timestamp":1700187407000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.20117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,23]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1002\/ajh.20117"],"URL":"https:\/\/doi.org\/10.1002\/ajh.20117","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,23]]}}}