{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T02:14:52Z","timestamp":1769825692505,"version":"3.49.0"},"reference-count":5,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T00:00:00Z","timestamp":1090540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucose\u20106\u2010phosphate dehydrogenase (G6PD) deficiency is the most common human enzymopathy, affecting more than 200 million people worldwide. To date more than 123 mutations in the G6PD gene have been discovered, among which 12 point mutations are found in the Chinese. Setting up a simple and accurate method for detecting these mutations is not only useful for diagnosing G6PD deficiency under some circumstances that it is difficult to measure the activity of the enzyme, but also for studying the frequency of the G6PD genotypes. The purpose of this study was to develop a simple, inexpensive and accurate method for detecting these common mutations. Microarray\u2010based assay was described in this study. Samples from 198 G6PD\u2010deficient persons were investigated. The DNA sequencing data supported the results obtained by microarray\u2010based assay. Thus, we concluded that the microarray\u2010based assay is a rapid, simple, inexpensive, and accurate method for detecting the most common G6PD gene mutations among the Chinese. This method involves the selective amplification of human G6PD gene with specific oligonucleotide primers, fragmentation and labeling of PCR products, followed by hybridization with allele\u2010specific oligonucleotide (ASO) probes on chip. Am. J. Hematol. 76:405\u2013412, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.20126","type":"journal-article","created":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T17:04:41Z","timestamp":1090602281000},"page":"405-412","source":"Crossref","is-referenced-by-count":17,"title":["Rapid detection of common Chinese glucose\u20106\u2010phosphate dehydrogenase (G6PD) mutations by microarray\u2010based assay"],"prefix":"10.1002","volume":"76","author":[{"given":"Ye","family":"Bang\u2010Ce","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu","family":"Hongqiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhensong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(91)90465-Q"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.11.3613.bloodjournal84113613"},{"key":"e_1_2_1_4_2","first-page":"1","article-title":"Standardization of techniques for the study of G6PD","volume":"366","author":"World Health Organization","year":"1967","journal-title":"WHO Tech Rep Ser"},{"key":"e_1_2_1_5_2","volume-title":"Manual of biochemical methods","author":"Beutler E","year":"1971"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.24.5456"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.20126","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.20126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T02:16:56Z","timestamp":1700187416000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.20126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,23]]},"references-count":5,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1002\/ajh.20126"],"URL":"https:\/\/doi.org\/10.1002\/ajh.20126","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,23]]}}}