{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T06:54:58Z","timestamp":1700031298583},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T00:00:00Z","timestamp":1164672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clonal T\u2010cell receptor (TCR) gamma and delta gene rearrangements were studied in 40 T\u2010ALL cases (pediatrics, 29; adults, 11) using PCR with homo\u2010heteroduplex analysis. At least one clonal TCRG or TCRD rearrangement was detected in 34 (85%) cases. TCR gamma (<jats:italic>TCRG<\/jats:italic>) rearrangement was detected in 25 (62.5%) cases that included 16 (55%) pediatrics and 9 (81.8%) adults. TCR delta (T<jats:italic>CRD<\/jats:italic>) rearrangement was detected in 14\/40 (35%) cases, which included 12 (41%) pediatrics and 2 (18%) adults. The frequency of V\u03b3I\u2010J\u03b31.3\/2.3 was significantly more in adults than pediatrics (81.8% vs. 41.3%, <jats:italic>P<\/jats:italic> = 0.02). In <jats:italic>TCRD<\/jats:italic>, V\u03b41\u2010J\u03b41 was rearranged in 10 (25%) cases. The surface membrane CD3 positive cases are significantly associated with absence of TCRD rearrangements (surface membrane CD3+ TCR\u03b4\u2212 84% vs. surface membrane CD3\u2212 TCR\u03b4\u2212 48%, <jats:italic>P<\/jats:italic> value = 0.03). Junctional region sequence analyzed with 10 cases each, of <jats:italic>TCRG<\/jats:italic> and <jats:italic>TCRD<\/jats:italic>, revealed an average junctional region of 7.4 nucleotides (range 2\u201318 nucleotides) in <jats:italic>TCRG<\/jats:italic> and 27 nucleotides (range 14\u201342 nucleotides) in <jats:italic>TCRD\u2010<\/jats:italic>complete rearrangements. In <jats:italic>TCRG<\/jats:italic>, trimming at the ends of V\u03b3 and J\u03b3 germline nucleotides resulted in deletion, on an average of 9.2 nucleotides. In <jats:italic>TCRD<\/jats:italic>, deletion of nucleotides of the V\u03b4 and J\u03b4 gene segments on an average was 3.5 nucleotides. The junctional region of <jats:italic>TCRD<\/jats:italic> is more diverse than <jats:italic>TCRG;<\/jats:italic> nevertheless, the frequency of <jats:italic>TCRG<\/jats:italic> was more than that of <jats:italic>TCRD<\/jats:italic> and hence we rely more on <jats:italic>TCRG<\/jats:italic> clonal markers to quantitate the minimal residual disease in T\u2010ALL. Am. J. Hematol., 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.20792","type":"journal-article","created":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T20:33:16Z","timestamp":1164745996000},"page":"215-221","source":"Crossref","is-referenced-by-count":4,"title":["T\u2010cell receptor gamma and delta gene rearrangements and junctional region characteristics in south Indian patients with T\u2010cell acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"82","author":[{"given":"Natarajan","family":"Sudhakar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirmala Karunakaran","family":"Nancy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamalalayam 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