{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:16:03Z","timestamp":1791141363180,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,5,29]],"date-time":"2007-05-29T00:00:00Z","timestamp":1180396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The clinical heterogeneity that characterizes chronic lymphocytic leukemia (CLL) poses critical questions concerning the identification of high risk patients. Unmutated IgV<jats:sub>H<\/jats:sub> genes, CD38 and ZAP\u201070 expression have emerged as the most useful tools in identifying aggressive CLL. The simultaneous expression of ZAP\u201070 and CD38 in 157 patients with CLL has been evaluated. Fifty\u2010seven patients (36%) were positive for ZAP\u201070 and 46 patients (29%) were positive for CD38. Both molecules were highly correlated and predictive of the clinical course of the disease. According to the simultaneous evaluation of ZAP\u201070 and CD38, patients were divided into three groups. In 81 patients (52%), there was a <jats:italic>negative concordance<\/jats:italic> of both molecules (ZAP\u201070<jats:sup>\u2212<\/jats:sup>\/CD38<jats:sup>\u2212<\/jats:sup>); in 27 patients (17%) there was a <jats:italic>positive concordance<\/jats:italic> (ZAP\u201070<jats:sup>+<\/jats:sup>\/CD38<jats:sup>+<\/jats:sup>); in 49 patients (31%) there was a <jats:italic>discordant expression<\/jats:italic> (ZAP\u201070<jats:sup>+<\/jats:sup>\/CD38<jats:sup>\u2212<\/jats:sup> and ZAP\u201070<jats:sup>\u2212<\/jats:sup>\/CD38<jats:sup>+<\/jats:sup>). A comparison of the clinical and laboratory data showed in ZAP\u201070<jats:sup>+<\/jats:sup>\/CD38<jats:sup>+<\/jats:sup> patients a significantly higher bone marrow and peripheral blood lymphocytosis, lower hemoglobin levels, more advanced clinical stage, and higher number of unmutated IgV<jats:sub>H<\/jats:sub> status with respect to the other two groups. Furthermore, ZAP\u201070<jats:sup>+<\/jats:sup>\/CD38<jats:sup>+<\/jats:sup> patients displayed a much shorter treatment\u2010free interval (median 12 months vs 42 months in <jats:italic>discordant<\/jats:italic> patients and not reached in ZAP\u201070<jats:sup>\u2212<\/jats:sup>CD38<jats:sup>\u2212<\/jats:sup> patients). These results prove that the concomitant evaluation of ZAP\u201070 and CD38 expression allows the separation of CLL patients in prognostic subgroups and suggest that their simultaneous assessment should become an integral component of the CLL diagnostic grid. Am. J. Hematol., 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.20936","type":"journal-article","created":{"date-parts":[[2007,5,29]],"date-time":"2007-05-29T18:50:47Z","timestamp":1180464647000},"page":"787-791","source":"Crossref","is-referenced-by-count":34,"title":["Prognostic significance of combined analysis of ZAP\u201070 and CD38 in chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"82","author":[{"given":"Giovanni","family":"D'Arena","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Tarnani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Rumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Vaisitti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Semra","family":"Aydin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaria","family":"De Filippi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Perrone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Chiusolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Deaglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Malavasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Laurenti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041720"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-07-2281"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1182\/blood.V94.6.1840","article-title":"IgV gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia","volume":"94","author":"Damle RN","year":"1999","journal-title":"Blood"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1848"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.1.181"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.9.2633"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428190109097682"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00049-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402339"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.3.1023"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3306"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa023143"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403147"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15260-9"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040857"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403707"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21437"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdj120"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-4986"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200012283432602"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-04-1677"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/09638280500260079"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.2005.00703.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-03-0989"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-10-3873"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-01-013003"},{"issue":"1","key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"959a","DOI":"10.1182\/blood.V104.11.959.959","article-title":"CD38 ligation in B\u2010chronic lymphocytic leukemia cells induce sequential tyrosine phosphorilation of ZAP\u201070, PLC\u2010g2 and ERK1\/2 proteins","volume":"104","author":"Deaglio S","year":"2004","journal-title":"Blood"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2669"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.20936","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.20936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T17:59:54Z","timestamp":1699984794000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.20936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,6]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/ajh.20936"],"URL":"https:\/\/doi.org\/10.1002\/ajh.20936","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,6]]}}}