{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:39:51Z","timestamp":1792737591259,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,6]],"date-time":"2012-05-06T00:00:00Z","timestamp":1336262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The direct antiglobulin test (DAT) is a laboratory test that detects immunoglobulin and\/or complement on the surface of red blood cells. The utility of the DAT is to sort hemolysis into an immune or nonimmune etiology. As with all tests, DAT results must be viewed in light of clinical and other laboratory data. This review highlights the most common clinical situations where the DAT can help classify causes of hemolysis, including autoimmune hemolytic anemia, transfusion\u2010related hemolysis, hemolytic disease of the fetus\/newborn, drug\u2010induced hemolytic anemia, passenger lymphocyte syndrome, and DAT\u2010negative hemolytic anemia. In addition, the pitfalls and limitations of the test are addressed. False reactions may occur with improper technique, including improper washing, centrifugation, and specimen agitation at the time of result interpretation. Patient factors, such as spontaneous red blood cell agglutination, may also contribute to false results. Am. J. Hematol. 87:707\u2013709, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23218","type":"journal-article","created":{"date-parts":[[2012,4,4]],"date-time":"2012-04-04T16:10:58Z","timestamp":1333555858000},"page":"707-709","source":"Crossref","is-referenced-by-count":112,"title":["The direct antiglobulin test: A critical step in the evaluation of hemolysis"],"prefix":"10.1002","volume":"87","author":[{"given":"Nicole D.","family":"Zantek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Koepsell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Daryl R.","family":"Tharp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia S.","family":"Cohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,6]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"49","article-title":"Neue Tatsachen uber die Blutkorperchen\u2010Agglutination","volume":"46","author":"Moreschi C","year":"1908","journal-title":"Zentralbl Bakteriol Parasitenkd Infektkr 1 Originale"},{"key":"e_1_2_5_3_2","first-page":"255","article-title":"A new test for the detection of weak and incomplete Rh agglutinins","volume":"26","author":"Coombs RR","year":"1945","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(87)70006-6"},{"key":"e_1_2_5_5_2","first-page":"25","volume-title":"Current Applications and Interpretations of the Direct Antiglobulin Test","author":"South SF","year":"1988"},{"key":"e_1_2_5_6_2","first-page":"3","article-title":"Replicate tests for the detection and correction of errors in anti\u2010human globulin (AHG) tests: optimum conditions and quality control","volume":"21","author":"Voak D","year":"1988","journal-title":"Haematologia (Budap)"},{"key":"e_1_2_5_7_2","unstructured":"AllanJ GarratyG.Positive direct antiglobulin tests in normal blood donors [abstract]. 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