{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:10:00Z","timestamp":1761844200362},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,9,11]],"date-time":"2012-09-11T00:00:00Z","timestamp":1347321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies concerning <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA levels by quantitative real\u2010time RT\u2010PCR (Q\u2010PCR) for chronic myelogenous leukemia (CML) have shown a significant concordance between peripheral blood (PB) and bone marrow (BM) assays. The objective of this study was to determine whether molecular monitoring using PB was comparable to using BM for CML. A comparative study was performed that analyzed the Q\u2010PCR results of 712 simultaneous PB and BM samples from 330 patients before and during imatinib therapy. For the 78 paired pretreatment samples, the level of <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA in PB was lower than that in BM (<jats:italic>P<\/jats:italic> = 0.007). Although the overall amounts of <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA in the PB and BM were comparable (<jats:italic>P<\/jats:italic>= 0.072) and there was a strong correlation (<jats:italic>r<\/jats:italic> = 0.839, <jats:italic>P<\/jats:italic> &lt; 0.001) with the 634 paired on\u2010treatment samples, the depth of the molecular response in PB was lower than that in BM (<jats:italic>P<\/jats:italic> &lt; 0.001). The level of <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA in PB was lower than that in BM where the BM <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA &lt; 1 log reduction (<jats:italic>P<\/jats:italic> &lt; 0.001) or \u2265 1\u2013&lt; 2 log\u2010reductions (<jats:italic>P<\/jats:italic> = 0.008) from the baseline, and higher than that where the BM <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA \u2265 2 log\u2010reductions (<jats:italic>P<\/jats:italic> &lt; 0.001). A strong correlation (<jats:italic>r<\/jats:italic> = 0.811, <jats:italic>P<\/jats:italic> &lt; 0.001) was only found where the BM <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA &lt; 1 log reduction. We conclude that the differences and correlations of <jats:italic>BCR\u2010ABL<\/jats:italic> mRNA between PB and BM assays depend on the depth of the molecular response in BM for CML during imatinib therapy. Am. J. Hematol., 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23321","type":"journal-article","created":{"date-parts":[[2012,8,3]],"date-time":"2012-08-03T18:00:36Z","timestamp":1344016836000},"page":"1065-1069","source":"Crossref","is-referenced-by-count":11,"title":["The differences and correlations of <i>BCR\u2010ABL<\/i> transcripts between peripheral blood and bone marrow assays are associated with the molecular responses in the bone marrow for chronic myelogenous leukemia"],"prefix":"10.1002","volume":"87","author":[{"given":"Qian","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Yu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Zhen","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Rong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue\u2010Yun","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Jun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,9,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa062867"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.38"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1002315"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0912614"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-273979"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.38.6565"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.25.0779"},{"key":"e_1_2_6_9_2","unstructured":"National Comprehensive Cancer Network clinical practice guidelines in oncology: chronic myelogenous leukemia version 2.2012.http:\/\/www.nccn.org\/NCCN Guidelines\u2122 & Clinical Resources\/ (enter in 20 Aug 2011)."},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404388"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403158"},{"issue":"1","key":"e_1_2_6_12_2","first-page":"160","article-title":"Quantitative polymerase chain reaction monitoring of BCR\u2010ABL during therapy with imatinib mesylate (STI571; 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