{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:47:31Z","timestamp":1788472051151,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T00:00:00Z","timestamp":1363305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>Cobalamin (vitamin B12) deficiency is a common cause of megaloblastic anemia in Western populations. Laboratory evaluation of megaloblastic anemia frequently includes the assessment of patient cobalamin and folate status. Current total serum cobalamin measurements are performed in the clinical laboratory with competitive binding luminescence assays, whose results may not always accurately reflect actual cobalamin stores. Surrogate markers of cobalamin deficiency such as methylmalonic acid and homocysteine have been utilized to improve diagnostic accuracy; however, the specificity of these tests by themselves is rather low. Measurement of the biologically active fraction of cobalamin, holotranscobalamin, has been proposed as a replacement for current total cobalamin assays. Although holotranscobalamin measurements appear to have slighter better sensitivity, the specificity of this assay remains to be determined. The relative merits and demerits of commonly available methods to assess cobalamin deficiency in patients with suspected megaloblastic anemia are discussed. Am. J. Hematol. 88:522\u2013526, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23421","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T08:34:59Z","timestamp":1361349299000},"page":"522-526","source":"Crossref","is-referenced-by-count":54,"title":["Laboratory testing for cobalamin deficiency in megaloblastic anemia"],"prefix":"10.1002","volume":"88","author":[{"given":"Matthew J.","family":"Oberley","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public Health Madison WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David T.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public Health Madison WI"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,15]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2009.09.006"},{"key":"e_1_2_14_3_1","volume-title":"Williams Hematology","author":"Green R","year":"2010"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197111182852103"},{"key":"e_1_2_14_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1182\/blood.V33.1.1.1","article-title":"Deficiency of vitamin B12\u2010binding alpha globulin in two brothers","volume":"33","author":"Carmel R","year":"1969","journal-title":"Blood"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-040253"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.161.12.1534"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.17.1.14"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197810122991506"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.013441"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197810122991501"},{"key":"e_1_2_14_12_1","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1093\/clinchem\/46.12.2017","article-title":"Failure of assay to identify low cobalamin concentrations","volume":"46","author":"Carmel R","year":"2000","journal-title":"Clin Chem"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.059055"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.333.7569.654-c"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1201655"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1204070"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-11-315564"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2009.178657"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1641"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-0971"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0b013e3283505852"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1373\/49.8.1367"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-2712(01)00003-8"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/48.3.852"},{"key":"e_1_2_14_25_1","first-page":"75","article-title":"Cobalamin deficiency in patients infected with the human immunodeficiency virus","volume":"36","author":"Remacha AF","year":"1999","journal-title":"Semin Hematol"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000041064"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045979"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(94)90149-X"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000314841"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.174516"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.013458"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2007.096784"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.061382"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2011.011039"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.361"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2010.158154"},{"key":"e_1_2_14_37_1","first-page":"231","article-title":"The limited value of methylmalonic acid, homocysteine and holotranscobalamin in the diagnosis of early B12 deficiency","volume":"91","author":"Goringe A","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-010-5764-4"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.23421","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.23421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T01:30:54Z","timestamp":1700616654000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.23421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,15]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/ajh.23421"],"URL":"https:\/\/doi.org\/10.1002\/ajh.23421","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,15]]}}}