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In this study, we investigated whether the specific microRNAs were related to the tumor behaviors in NNKTL. MiRNA array and Quantitative RT\u2010PCR analyses revealed that miR\u201015a was expressed at a much lower level in NNKTL cells (SNK\u20101, SNK\u20106, and SNT\u20108) than in normal peripheral NK cells and EBV\u2010negative NK cell line KHYG\u20101. Quantitative PCR and western blot analyses showed that the expression of MYB and cyclin D1, which are validated targets of miR\u201015a, was higher in NNKTL cells. Transfection of NNKTL cells (SNK\u20106 and SNT\u20108) with a miR\u201015a precursor decreased MYB and cyclin D1 levels, thereby blocking G1\/S transition and cell proliferation. Knockdown of EBV\u2010encoded latent membrane protein 1 (LMP1) significantly increased miR\u201015a expression in SNK\u20106 cells. In NNKTL tissues, we found that reduced miR\u201015a expression, which correlated with MYB and cyclin D1 expression, was associated with poor prognosis of NNKTL patients. These data suggest that downregulation of miR\u201015a, possibly due to LMP1, implicates in the pathogenesis of NNKTL by inducing cell proliferation via MYB and cyclin D1. Thus, miR\u201015a could be a potential target for antitumor therapy and a prognostic predictor for NNKTL. Am. J. Hematol. 89:25\u201333, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23570","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T12:42:44Z","timestamp":1377002564000},"page":"25-33","source":"Crossref","is-referenced-by-count":43,"title":["Downregulation of miR\u201015a due to LMP1 promotes cell proliferation and predicts poor prognosis in nasal NK\/T\u2010cell lymphoma"],"prefix":"10.1002","volume":"89","author":[{"given":"Yuki","family":"Komabayashi","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Asahikawa Medical University, Asahikawa, Midorigaoka\u2010higashi 2\u20101\u20101\u20101 Asahikawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kan","family":"Kishibe","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Asahikawa Medical University, Asahikawa, Midorigaoka\u2010higashi 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