{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:52:00Z","timestamp":1790614320718,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T00:00:00Z","timestamp":1384905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>This study evaluates the functional procoagulant features of plasma microparticle (MP) to explore the MP contribution to the hypercoagulable state of patients with essential thrombocythemia (ET). Platelet\u2010free plasma samples were obtained from 73 ET patients (37 positive for the JAK2V617F mutation) and 72 control subjects. The calibrated automated thrombogram (CAT) was performed in plasma samples to determine thrombin generation of MP\u2010associated tissue factor (TF) and procoagulant phospholipid (PPL) activity, and the STA Procoag PPL assay to measure MP\u2010PPL activity only. Both thrombin generation and PPL procoagulant activities were found significantly elevated in ET patients compared to controls, and were associated to significantly higher levels of TF antigen and FVIIa\/AT complex. Thrombin generation was significantly greater in JAK2\u2010V617F positive compared to JAK2\u2010V617F negative patients and normal subjects. Significant correlations were found between the PPL\u2010assay and the different parameters of the CAT assay. No difference was seen between the thrombosis and no thrombosis group. Prospective studies are needed to test whether MP\u2010associated thrombin generation and procoagulant activity may predict for thrombosis in these patients. Am. J. Hematol. 89:68\u201373, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23590","type":"journal-article","created":{"date-parts":[[2013,9,5]],"date-time":"2013-09-05T12:35:16Z","timestamp":1378384516000},"page":"68-73","source":"Crossref","is-referenced-by-count":56,"title":["Phospholipid\u2010dependent procoagulant activity is highly expressed by circulating microparticles in patients with essential thrombocythemia"],"prefix":"10.1002","volume":"89","author":[{"given":"Marina","family":"Marchetti","sequence":"first","affiliation":[{"name":"Division of Immunohematology and Transfusion Medicine Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen J.","family":"Tartari","sequence":"additional","affiliation":[{"name":"Division of Immunohematology and Transfusion Medicine Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Russo","sequence":"additional","affiliation":[{"name":"Division of Immunohematology and Transfusion Medicine Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Panova\u2010Noeva","sequence":"additional","affiliation":[{"name":"Division of Immunohematology and Transfusion Medicine Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annamaria","family":"Leuzzi","sequence":"additional","affiliation":[{"name":"Division of Immunohematology and Transfusion Medicine Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Rambaldi","sequence":"additional","affiliation":[{"name":"Division of Hematology Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Finazzi","sequence":"additional","affiliation":[{"name":"Division of Hematology Hospital Papa Giovanni XXIII Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Woodhams","sequence":"additional","affiliation":[{"name":"HaemaCon, Ltd. 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