{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:16:32Z","timestamp":1764332192249},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T00:00:00Z","timestamp":1393459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>For decades now, it is well established that chronic myeloid leukemia (CML) is a hematopoietic stem cell (HPC) disorder. However, it remains to be determined whether <jats:italic>BCR\u2010ABL1<\/jats:italic> gene rearrangement occurs in a HPC or at an earlier stem cell and whether the degree of involvement of hematopoiesis by the <jats:italic>BCR\u2010ABL1<\/jats:italic> fusion gene relates to the response to therapy. Here, we have investigated by interphase fluorescence in situ hybridization (iFISH) the distribution of <jats:italic>BCR\u2010ABL1<\/jats:italic> fusion gene in FACS\u2010sorted bone marrow (BM) populations of mesenchymal precursor cells (MPC) and other hematopoietic cell populations from 18 newly diagnosed CML patients. Overall, our results showed systematic involvement at relatively high percentages of BM maturing neutrophils (97%\u2009\u00b1\u200915%), basophils (95%\u2009\u00b1\u200912%), eosinophils (90%\u2009\u00b1\u20098%), CD34<jats:sup>+<\/jats:sup> precursors cells (90%\u2009\u00b1\u20097%), monocytes (84%\u2009\u00b1\u200930%), nucleated red blood cells (87%\u2009\u00b1\u200924%), and mast cells (77%\u2009\u00b1\u200933%). By contrast, MPC (30%\u2009\u00b1\u200934%), B\u2010cells (15%\u2009\u00b1\u200927%), T\u2010lymphocytes (50%\u2009\u00b1\u200926%), and NK\u2010cells (35%\u2009\u00b1\u200934%) were involved at lower percentages. In 8\/18 CML patients, \u22652 tumor <jats:italic>BCR\u2010ABL1<jats:sup>+<\/jats:sup><\/jats:italic> subclones were detected by iFISH. Of note, all tumor cell subclones were systematically detected in CD34<jats:sup>+<\/jats:sup> cells, whereas MPC were only involved by the ancestral tumor cell subclone. In summary, here we confirm the presence at diagnosis of the <jats:italic>BCR\u2010ABL1<\/jats:italic> fusion gene in MPC, CD34<jats:sup>+<\/jats:sup> precursors, and other different BM hematopoietic myeloid cell lineages from CML patients, including also in a significant fraction of cases, a smaller percentage of T, B, and NK lymphocytes. Interestingly, involvement of MPC was restricted to the ancestral <jats:italic>BCR\u2010ABL1<\/jats:italic><jats:sup>+<\/jats:sup> subclone. Am. J. Hematol. 89:288\u2013294, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23626","type":"journal-article","created":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T12:35:56Z","timestamp":1383914156000},"page":"288-294","source":"Crossref","is-referenced-by-count":9,"title":["Involvement of primary mesenchymal precursors and hematopoietic bone marrow cells from chronic myeloid leukemia patients by <i>BCR\u2010ABL1<\/i> fusion gene"],"prefix":"10.1002","volume":"89","author":[{"given":"Mauricio","family":"Chandia","sequence":"first","affiliation":[{"name":"Servicio de Hematolog\u00eda IBSAL\u2010Hospital Universitario de Salamanca Paseo de San Vicente, 58\u2013182 37007 Salamanca Spain"},{"name":"Becario Fundaci\u00f3n Carolina\u2010Fundaci\u00f3n BBVA Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9-Mar\u00eda","family":"Sayagu\u00e9s","sequence":"additional","affiliation":[{"name":"Servicio General de 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