{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:38:57Z","timestamp":1792600737257,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,5,16]],"date-time":"2014-05-16T00:00:00Z","timestamp":1400198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>\n                    <jats:italic>CALR<\/jats:italic>\n                    (calreticulin) trails\n                    <jats:italic>JAK<\/jats:italic>\n                    2 as the second most mutated gene in essential thrombocythemia (ET). Mutant\n                    <jats:italic>CALR<\/jats:italic>\n                    in ET is a result of frameshift mutations, caused by exon 9 deletions or insertions; type\u20101, 52\u2010bp deletion (p.L367fs*46), and type\u20102, 5\u2010bp TTGTC insertion (p.K385fs*47) variants constitute more than 80% of these mutations. The current study includes a total of 1027 patients divided into test (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009402) and validation (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009625) cohorts. Among the 402 ET patients in the test cohort, 227 (57%) harbored\n                    <jats:italic>JAK<\/jats:italic>\n                    2, 11 (3%) Myeloproliferative leukemia virus oncogene (\n                    <jats:italic>MPL<\/jats:italic>\n                    ), and 114 (28%)\n                    <jats:italic>CALR<\/jats:italic>\n                    mutations; 12% were wild\u2010type for all three mutations (i.e., triple\u2010negative). Among the 114 patients with\n                    <jats:italic>CALR<\/jats:italic>\n                    mutations, 51 (45%) displayed type\u20101 and 44 (39%) type\u20102 variants; compared to mutant\n                    <jats:italic>JAK<\/jats:italic>\n                    2, both variants were associated with higher platelet and lower hemoglobin and leukocyte counts. However, male sex was associated with only type\u20101 (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.005) and younger age with type\u20102 (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.001) variants. Notably, platelet count was significantly higher in type\u20102 vs. type\u20101\n                    <jats:italic>CALR<\/jats:italic>\n                    \u2010mutated patients (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.03) and the particular observation was validated in the validation cohort that included 111\n                    <jats:italic>CALR<\/jats:italic>\n                    \u2010mutated ET patients (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.002). These findings, coupled with the recent demonstration of preferential expression of mutant and wild\u2010type\n                    <jats:italic>CALR<\/jats:italic>\n                    in megakaryocytes, suggest differential effects of\n                    <jats:italic>CALR<\/jats:italic>\n                    variants on thrombopoiesis. Am. J. Hematol. 89:E121\u2013E124, 2014. \u00a9 2014 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/ajh.23743","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T07:22:21Z","timestamp":1397892141000},"source":"Crossref","is-referenced-by-count":195,"title":["Type 1 versus Type 2 calreticulin mutations in essential thrombocythemia: A collaborative study of 1027 patients"],"prefix":"10.1002","volume":"89","author":[{"given":"Ayalew","family":"Tefferi","sequence":"first","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emnet A.","family":"Wassie","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Guglielmelli","sequence":"additional","affiliation":[{"name":"Laboratorio Congiunto MMPC Department of experimental and clinical medicine University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naseema","family":"Gangat","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alem A.","family":"Belachew","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terra L.","family":"Lasho","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christy","family":"Finke","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhett P.","family":"Ketterling","sequence":"additional","affiliation":[{"name":"Division of Cytogenetics Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Curtis A.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Animesh","family":"Pardanani","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra P.","family":"Wolanskyj","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Medicine Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margherita","family":"Maffioli","sequence":"additional","affiliation":[{"name":"Division of Hematology Genetic Unit, Medical Genetic and Cytogenetics Laboratory, SSD, SMEL, University Hospital Ospedale di Circolo e Fondazione Macchi Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Casalone","sequence":"additional","affiliation":[{"name":"Division of Hematology Genetic Unit, Medical Genetic and Cytogenetics Laboratory, SSD, SMEL, University Hospital Ospedale di Circolo e Fondazione Macchi Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Pacilli","sequence":"additional","affiliation":[{"name":"Laboratorio Congiunto MMPC Department of experimental and clinical medicine University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro M.","family":"Vannucchi","sequence":"additional","affiliation":[{"name":"Laboratorio Congiunto MMPC Department of experimental and clinical medicine University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Passamonti","sequence":"additional","affiliation":[{"name":"Division of Hematology Genetic Unit, Medical Genetic and Cytogenetics Laboratory, SSD, SMEL, University Hospital Ospedale di Circolo e Fondazione Macchi Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1311347"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1312542"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2014.16"},{"key":"e_1_2_6_5_1","unstructured":"TefferiA LashoTL FinkeCM et al. CALR vs JAK2 vs MPL\u2010mutated or triple\u2010negative myelofibrosis: Clinical cytogenetic and molecular comparisons. Leukemia in press. DOI 10.1038\/leu.2014.3."},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-539098"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-538983"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.119"},{"key":"e_1_2_6_9_1","unstructured":"TefferiA GuglielmelliP LashoTL et al. CALR and ASXL1 mutations\u2010based molecular prognostication in primary myelofibrosis: An international study of 570 patients. Leukemia in press."},{"key":"e_1_2_6_10_1","unstructured":"GuglielmelliP LashoTL RotunnoG et al. The number of prognostically detrimental mutations and prognosis in primary myelofibrosis: An international study of 797 patients. 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Leukemia in press."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2013.05.006"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.302184"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.23743","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.23743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T08:17:04Z","timestamp":1695457024000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.23743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,16]]},"references-count":18,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1002\/ajh.23743"],"URL":"https:\/\/doi.org\/10.1002\/ajh.23743","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,16]]}}}