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Lessell\u00e2\u20ac\u2122s Scholarship"],"award-info":[{"award-number":["J.M. Lessell\u00e2\u20ac\u2122s Scholarship"]}],"id":[{"id":"10.13039\/501100000332","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>This paper demonstrates the enrichment of reticulocytes by centrifuging whole blood through aqueous multiphase systems (AMPSs)\u2014immiscible phases of solutions of polymers that form step\u2010gradients in density. The interfaces of an AMPS concentrate cells; this concentration facilitates the extraction of blood enriched for reticulocytes. AMPS enrich reticulocytes from blood from both healthy and hemochromatosis donors. Varying the osmolality and density of the phases of AMPS provides different levels of enrichment and yield of reticulocytes. A maximum enrichment of reticulocytemia of 64\u2009\u00b1\u20093% was obtained from donors with hemochromatosis. When used on peripheral blood from normal donors, AMPS can provide a higher yield of enriched reticulocytes and a higher proportion of reticulocytes expressing CD71 than differential centrifugation followed by centrifugation over Percoll. Blood enriched for reticulocytes by AMPS could be useful for research on malaria. Several species of malaria parasites show a preference to invade young erythrocytes and reticulocytes; this preference complicates <jats:italic>in vitro<\/jats:italic> cultivation of these species in human blood. <jats:italic>Plasmodium knowlesi<\/jats:italic> malaria parasites invade normal human blood enriched for reticulocytes by AMPSs at a rate 2.2 times greater (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) than they invade unenriched blood. Parasite invasion in normal blood enriched by AMPS was 1.8 times greater (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) than in blood enriched to a similar reticulocytemia by differential centrifugation followed by centrifugation over Percoll. The enrichment of reticulocytes that are invaded by malaria parasites demonstrates that AMPSs can provide a label\u2010free method to enrich cells for biological research. Am. J. Hematol. 90:31\u201336, 2015. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.23860","type":"journal-article","created":{"date-parts":[[2014,9,27]],"date-time":"2014-09-27T08:58:35Z","timestamp":1411808315000},"page":"31-36","source":"Crossref","is-referenced-by-count":41,"title":["Enrichment of reticulocytes from whole blood using aqueous multiphase systems of polymers"],"prefix":"10.1002","volume":"90","author":[{"given":"Ashok A.","family":"Kumar","sequence":"first","affiliation":[{"name":"School of Engineering and Applied Sciences Harvard University Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caeul","family":"Lim","sequence":"additional","affiliation":[{"name":"Harvard School of Public Health Harvard University Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yovany","family":"Moreno","sequence":"additional","affiliation":[{"name":"Harvard School of Public Health Harvard University 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