{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:17:13Z","timestamp":1792804633808,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T00:00:00Z","timestamp":1422835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Laboratory of Excellence GR-Ex","award":["ANR-11-LABX-0051"],"award-info":[{"award-number":["ANR-11-LABX-0051"]}],"id":[{"id":"https:\/\/ror.org\/00ezvft90","id-type":"ROR","asserted-by":"crossref"}]},{"name":"\u201cInvestissements d'avenir\u201d of the French National Research Agency","award":["ANR-11-IDEX-0005-02"],"award-info":[{"award-number":["ANR-11-IDEX-0005-02"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Institute d'Alembert (Guidecells Project); LASIPS Labex (SIMULOS Project); DIM MalInf; the Gates Foundation; Follereau Foundation","id":[{"id":"https:\/\/ror.org\/0456r8d26","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>\n                    Red blood cells (RBCs) are deformable and flow through vessels narrower than their own size. Their deformability is most stringently challenged when they cross micrometer\u2010wide slits in the spleen. In several inherited or acquired RBC disorders, blockade of small vessels by stiff RBCs can trigger organ damage, but a functional spleen is expected to clear these abnormal RBCs from the circulation before they induce such complications. We analyzed flow behavior of RBCs in a microfluidic chip that replicates the mechanical constraints imposed on RBCs as they cross the human spleen. Polymer microchannels obtained by soft lithography with a hydraulic diameter of 25 \u03bcm drove flow into mechanical filtering units where RBCs flew either slowly through 5\u2010 to 2\u2010\u03bcm\u2010wide slits or rapidly along 10\u2010\u03bcm\u2010wide channels, these parallel paths mimicking the splenic microcirculation. Stiff heated RBCs accumulated in narrow slits seven times more frequently than normal RBCs infused simultaneously. Stage\u2010dependent retention of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    \u2010infected RBCs was also observed in these slits. We also analyzed RBCs from patients with hereditary spherocytosis and observed retention for those having the most altered mechanical properties as determined by ektacytometry. Thus, in keeping with previous observations\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>ex vivo<\/jats:italic>\n                    , the chip successfully discriminated poorly deformable RBCs based on their distinct mechanical properties and on the intensity of the cell alteration. Applications to the exploration of the pathogenesis of malaria, hereditary spherocytosis, sickle cell disease and other RBC disorders are envisioned.Am. J. Hematol. 90:339\u2013345, 2015. \u00a9 2015 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/ajh.23941","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T02:53:53Z","timestamp":1421031233000},"page":"339-345","source":"Crossref","is-referenced-by-count":98,"title":["A biomimetic microfluidic chip to study the circulation and mechanical retention of red blood cells in the spleen"],"prefix":"10.1002","volume":"90","author":[{"given":"Julien","family":"Picot","sequence":"first","affiliation":[{"name":"Institut National De La Transfusion Sanguine Paris F\u201075739 France"},{"name":"Inserm, UMR_S1134 Paris France F\u201075739"},{"name":"Universit\u00e9 Paris Diderot Sorbonne Paris Cit\u00e9 Paris France"},{"name":"Laboratory of Excellence GR\u2010Ex Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Papa Alioune","family":"Ndour","sequence":"additional","affiliation":[{"name":"Laboratory of Excellence 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