{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:44:52Z","timestamp":1790426692605,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T00:00:00Z","timestamp":1441843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009882","name":"Regione Lombardia","doi-asserted-by":"crossref","award":["ISAV 2011 (to C.G.P.)"],"award-info":[{"award-number":["ISAV 2011 (to C.G.P.)"]}],"id":[{"id":"10.13039\/501100009882","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"crossref","award":["AIRC 2013 IG-14249 (to C.G.P.)"],"award-info":[{"award-number":["AIRC 2013 IG-14249 (to C.G.P.)"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>Imatinib is effective for the treatment of chronic myeloid leukemia (CML). However even undetectable BCR\u2010ABL1 by Q\u2010RT\u2010PCR does not equate to eradication of the disease. Digital\u2010PCR (dPCR), able to detect 1 BCR\u2010ABL1 positive cell out of 10<jats:sup>7<\/jats:sup>, has been recently developed. The ISAV study is a multicentre trial aimed at validating dPCR to predict relapses after imatinib discontinuation in CML patients with undetectable Q\u2010RT\u2010PCR. CML patients under imatinib therapy since more than 2 years and with undetectable PCR for at least 18 months were eligible. Patients were monitored by standard Q\u2010RT\u2010PCR for 36 months. Patients losing molecular remission (two consecutive positive Q\u2010RT\u2010PCR with at least 1 BCR\u2010ABL1\/ABL1 value above 0.1%) resumed imatinib. The study enrolled 112 patients, with a median follow\u2010up of 21.6 months. Fifty\u2010two of the 108 evaluable patients (48.1%), relapsed; 73.1% relapsed in the first 9 months but 14 late relapses were observed between 10 and 22 months. Among the 56 not\u2010relapsed patients, 40 (37.0% of total) regained Q\u2010RT\u2010PCR positivity but never lost MMR. dPCR results showed a significant negative predictive value ratio of 1.115 [95% CI: 1.013\u20131.227]. An inverse relationship between patients age and risk of relapse was evident: 95% of patients &lt;45 years relapsed versus 42% in the class \u226545 to &lt;65 years and 33% of patients \u226565 years [<jats:italic>P<\/jats:italic>(\u03c7<jats:sup>2<\/jats:sup>)\u2009&lt;\u20090.0001]. Relapse rates ranged between 100% (&lt;45 years, dPCR+) and 36% (&gt;45 years, dPCR\u2010). Imatinib can be safely discontinued in the setting of continued PCR negativity; age and dPCR results can predict relapse. Am. J. Hematol. 90:910\u2013914, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ajh.24120","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T14:13:38Z","timestamp":1436969618000},"page":"910-914","source":"Crossref","is-referenced-by-count":195,"title":["Age and d<scp>PCR<\/scp> can predict relapse in <scp>CML<\/scp> patients who discontinued imatinib: The <scp>ISAV<\/scp> study"],"prefix":"10.1002","volume":"90","author":[{"given":"Silvia","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Health Sciences University Of Milano\u2010Bicocca Monza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Vagge","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University Of Milano\u2010Bicocca Monza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"le Coutre","sequence":"additional","affiliation":[{"name":"Department of Haematology And Oncology Charit\u00e9 \u2010 Humboldt\u2010Universit\u00e4t Campus Virchow Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Abruzzese","sequence":"additional","affiliation":[{"name":"Department of Haematology S. 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