{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T03:20:24Z","timestamp":1773717624366,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T00:00:00Z","timestamp":1493683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dan and Betty Kahn Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myeloproliferative neoplasms (MPNs) are clonal hematopoietic stem cell (HSC) disorders that can be classified on the basis of genetic, clinical, phenotypic features. Genetic lesions such as JAK2 mutations and BCR\u2010ABL translocation are often mutually exclusive in MPN patients and lead to essential thrombocythemia, polycythemia vera, or myelofibrosis or chronic myeloid leukemia, respectively. Nevertheless, coexistence of these genetic aberrations in the same patient has been reported. Whether these aberrations occur in the same stem cell or a different cell is unclear, but an unstable genome in the HSCs seems to be the common antecedent. In an effort to characterize the underlying genetic events that might contribute to the appearance of more than one MPN in a patient, we studied neoplastic cells from patients with dual MPNs by next\u2010generation sequencing. We observed that most patients with two MPNs harbored mutations in genes known to contribute to clonal hematopoiesis through altered epigenetic regulation such as TET2, ASXL1\/2, SRSF2, and IDH2 at varying frequencies (1%\u201347%). In addition, we found that some patients also harbored oncogenic mutations in N\/KRAS, TP53, BRAF, EZH2, and GNAS at low frequencies, which probably represent clonal evolution. These findings support the hypothesis that hematopoietic cells from MPN patients harbor multiple genetic aberrations, some of which can contribute to clonal dominance. Acquiring mutations in JAK2\/CALR\/MPL or the BCR\u2010ABL translocation probably drive the oncogenic phenotype towards a specific MPN. Further, we propose that the acquisition of BCR\u2010ABL in these patients is frequently a secondary event resulting from an unstable genome.<\/jats:p>","DOI":"10.1002\/ajh.24728","type":"journal-article","created":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T11:14:01Z","timestamp":1490354041000},"page":"555-561","source":"Crossref","is-referenced-by-count":13,"title":["Clinical characteristics and whole exome\/transcriptome sequencing of coexisting chronic myeloid leukemia and myelofibrosis"],"prefix":"10.1002","volume":"92","author":[{"given":"Malathi","family":"Kandarpa","sequence":"first","affiliation":[{"name":"Department of Internal Medicine University of Michigan Comprehensive Cancer Center Ann Arbor Michigan 48109 USA"},{"name":"Division of Hematology\/Oncology University of Michigan Comprehensive Cancer Center Ann Arbor Michigan 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Mi","family":"Wu","sequence":"additional","affiliation":[{"name":"Michigan Center for Translational Pathology, University of Michigan Medical School Ann Arbor Michigan 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Robinson","sequence":"additional","affiliation":[{"name":"Michigan Center for Translational Pathology, University of Michigan Medical School Ann Arbor Michigan 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick William","family":"Burke","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Michigan Comprehensive Cancer Center Ann Arbor Michigan 48109 USA"},{"name":"Division of Hematology\/Oncology University of Michigan Comprehensive Cancer Center Ann Arbor Michigan 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arul M.","family":"Chinnaiyan","sequence":"additional","affiliation":[{"name":"Michigan Center for Translational Pathology, University of Michigan Medical School Ann Arbor Michigan 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3361-3981","authenticated-orcid":false,"given":"Moshe","family":"Talpaz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Michigan Comprehensive Cancer Center Ann Arbor Michigan 48109 USA"},{"name":"Division of Hematology\/Oncology University of Michigan Comprehensive Cancer Center Ann Arbor Michigan 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2008.1.436"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1311347"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404955"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0300-1652.149177"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404993"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-11-467787"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.47.8669"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13932"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2405088"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.189621.115"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.10080"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-661983"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-537167"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2016.05.006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23652"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-07-516385"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-365007"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-014-2248-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2015.77"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1408617"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.130"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2015.02.035"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.045591"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.06.032"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-04-555508"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2012.668188"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008004"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2016.05.005"}],"container-title":["American 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