{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:35:18Z","timestamp":1783625718344,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T00:00:00Z","timestamp":1537920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The B\/T subtype of mixed phenotype acute leukemia (B\/T MPAL) is defined by co\u2010expression of antigens of both B\u2010 and T\u2010cell lineages on leukemic blasts. Although it has been suggested that multilineage antigen expression portends poor response to chemotherapy, the clinical characteristics and driver mutations that underlie the pathogenesis of this rare subtype of acute leukemia are scarcely known. We identified nine cases of B\/T MPAL from multiple institutions and correlated clinical and immunophenotypic findings with next\u2010generation sequencing data. We report that B\/T MPAL commonly presents with lymphadenopathy in adolescence and young adulthood. While the tumors have diverse cytogenetic and genomic perturbations, recurrent acquired aberrations include mutations in the putative transcriptional regulator <jats:italic>PHF6<\/jats:italic> and the JAK\u2010STAT and Ras signaling pathways. Alterations were also identified in genes encoding hematopoietic transcription factors, cell cycle regulators\/tumor suppressors, and chromatin modifying enzymes. The genomic landscape of B\/T MPAL strongly resembles that of T\u2010ALL subgroups associated with early developmental arrest, while genetic alterations that are common in B\u2010ALL were rarely seen. Two\u2010thirds of the patients responded to ALL\u2010based chemotherapy with or without stem cell transplantation. Our observations lay the groundwork for further study of the unique biology and clinical trajectory of  B\/T MPAL.<\/jats:p>","DOI":"10.1002\/ajh.25256","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T07:45:49Z","timestamp":1534491949000},"page":"1358-1367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Genomic and clinical characterization of B\/T mixed phenotype acute leukemia reveals recurrent features and T\u2010ALL like mutations"],"prefix":"10.1002","volume":"93","author":[{"given":"Xiaoli","family":"Mi","sequence":"first","affiliation":[{"name":"Harvard\u2010MIT Division of Health Sciences and Technology Harvard Medical School  Boston Massachusetts"},{"name":"Department of Pathology Boston Children's Hospital  Boston 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Memorial Sloan Kettering Cancer Center  New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail","family":"Roshal","sequence":"additional","affiliation":[{"name":"Hematopathology Service, Department of Pathology, Memorial Sloan Kettering Cancer Center  New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline S.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis B.","family":"Silverman","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology Dana\u2010Farber Cancer Institute  Boston Massachusetts"},{"name":"Division of Pediatric Hematology\u2010Oncology Boston Children's Hospital  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen 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