{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:13:34Z","timestamp":1784196814921,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T00:00:00Z","timestamp":1547078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>von Willebrand factor (VWF) ristocetin cofactor activity (VWF:RCo) by platelet aggregometry has been considered the gold standard for evaluating the ability of VWF to bind platelets for over 40\u2009years. Many automated systems no longer require platelets and rather rely on agglutination of latex particles. Automated methods of measuring VWF activity have improved performance characteristics and are performed on the same coagulation instruments used for routine testing via immunoturbidimetric methodology. Alternatively, a newer chemiluminescence assay system for measuring VWF activity demonstrates excellent performance characteristics. As these methods are becoming widely used, it is important to assess their performance in diagnosing and monitoring different types of von Willebrand disease. We review the automated methodologies and the published performance of these VWF assays. Advantages and limitations of these automated methods are discussed.<\/jats:p>","DOI":"10.1002\/ajh.25393","type":"journal-article","created":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T10:40:04Z","timestamp":1545993604000},"page":"496-503","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Automated assays for von Willebrand factor activity"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1489-5592","authenticated-orcid":false,"given":"Russell A.","family":"Higgins","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Texas Health Science Center San Antonio  San Antonio Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Goodwin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Larner College of Medicine, University of Vermont  Burlington Vermont"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,10]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.2.454.454"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02146.x"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24556"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12964"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1960.00270130099012"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1653684"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V41.5.687.687"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(73)90112-6"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/63.2.210"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107465"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPH5JK4ONENPAE"},{"key":"e_1_2_14_13_1","first-page":"178","article-title":"Lower limit of assay sensitivity: an under\u2010recognized and significant problem in von Willebrand disease identification and classification","volume":"21","author":"Favaloro EJ","year":"2008","journal-title":"Clin Lab Sci"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-947864"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2010.11.029"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.04123.x"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14145"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2015.01.027"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12264"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12087"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12203"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12380"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12937"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2015.12.010"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1309\/CEPND3LFHQ87XU4D"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mbc.0000233365.10981.08"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2011.02662.x"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029610379848"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04460.x"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10766"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12064"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12218"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/MBC.0000000000000169"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2014.09.024"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12437"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04729.x"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2014.04.033"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2017.09.025"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-10-249102"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-08-299016"},{"key":"e_1_2_14_41_1","first-page":"241","volume-title":"An Algorithmic Approach to Hemostasis Testing","author":"Van Cott EM","year":"2016"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2016.03.001"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13243"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.25393","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.25393","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ajh.25393","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.25393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T16:21:19Z","timestamp":1693930879000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.25393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,10]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/ajh.25393"],"URL":"https:\/\/doi.org\/10.1002\/ajh.25393","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,10]]},"assertion":[{"value":"2018-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}