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Although gene expression profiling and next generations sequencing have been proven to define specific features recurrently associated with distinct entities, genomic\u2010based stratifications have not yet led to definitive diagnostic criteria and\/or entered into the routine clinical practice. Herein, to improve the current molecular classification between AITL and PTCL\u2010NOS, we analyzed the transcriptional profiles from 503 PTCLs stratified according to their molecular configuration and integrated them with genomic data of recurrently mutated genes (<jats:italic>RHOA<\/jats:italic><jats:sup>\n<jats:italic>G17V<\/jats:italic><\/jats:sup>, <jats:italic>TET2<\/jats:italic>, <jats:italic>IDH2<\/jats:italic><jats:sup>\n<jats:italic>R172<\/jats:italic><\/jats:sup>, and <jats:italic>DNMT3A<\/jats:italic>) in 53 cases (39 AITLs and 14 PTCL\u2010NOSs) included in the series. Our analysis unraveled that the mutational status of <jats:italic>RHOA<\/jats:italic><jats:sup>\n<jats:italic>G17V<\/jats:italic><\/jats:sup>, <jats:italic>TET2,<\/jats:italic> and <jats:italic>DNMT3A<\/jats:italic> poorly correlated, individually, with peculiar transcriptional fingerprints. Conversely, in <jats:italic>IDH2<\/jats:italic><jats:sup>\n<jats:italic>R172<\/jats:italic><\/jats:sup> samples a strong transcriptional signature was identified that could act as a surrogate for mutational status. The integrated analysis of clinical, mutational, and molecular data led to a simplified 19\u2010gene signature that retains high accuracy in differentiating the main nodal PTCL entities. The expression levels of those genes were confirmed in an independent cohort profiled by RNA\u2010sequencing.<\/jats:p>","DOI":"10.1002\/ajh.25450","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T12:15:13Z","timestamp":1551701713000},"page":"628-634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Integration of transcriptional and mutational data simplifies the stratification of peripheral T\u2010cell lymphoma"],"prefix":"10.1002","volume":"94","author":[{"given":"Francesco","family":"Maura","sequence":"first","affiliation":[{"name":"Myeloma Service, Department of Medicine Memorial Sloan Kettering Cancer Center  New York New York"},{"name":"Cancer, Ageing and Somatic Mutation Programme Wellcome Trust Sanger Institute  Hinxton United Kingdom"},{"name":"Department of Oncology and Hemato\u2010Oncology University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0582-6170","authenticated-orcid":false,"given":"Luca","family":"Agnelli","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hemato\u2010Oncology University of Milan  Milan Italy"},{"name":"Hematology Foundation IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Leongamornlert","sequence":"additional","affiliation":[{"name":"Cancer, Ageing and Somatic Mutation Programme Wellcome Trust Sanger Institute  Hinxton United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niccol\u00f2","family":"Bolli","sequence":"additional","affiliation":[{"name":"Cancer, Ageing and Somatic Mutation Programme Wellcome Trust Sanger Institute  Hinxton United Kingdom"},{"name":"Department of Oncology and Hemato\u2010Oncology University of Milan  Milan Italy"},{"name":"Division of Hematology Fondazione IRCCS Istituto Nazionale dei Tumori  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wing C.","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Dodero","sequence":"additional","affiliation":[{"name":"Division of Hematology Fondazione IRCCS Istituto Nazionale dei Tumori  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1039-1757","authenticated-orcid":false,"given":"Cristiana","family":"Carniti","sequence":"additional","affiliation":[{"name":"Division of Hematology Fondazione IRCCS Istituto Nazionale dei Tumori  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayla B.","family":"Heavican","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center  Omaha Nebraska"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Pellegrinelli","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Fondazione IRCCS Istituto Nazionale dei Tumori  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Pruneri","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Fondazione IRCCS Istituto Nazionale dei Tumori  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Butler","sequence":"additional","affiliation":[{"name":"Cancer, Ageing and Somatic Mutation Programme Wellcome Trust Sanger Institute  Hinxton United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shriram G.","family":"Bhosle","sequence":"additional","affiliation":[{"name":"Cancer, Ageing and Somatic Mutation Programme Wellcome Trust Sanger Institute  Hinxton United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Chiappella","sequence":"additional","affiliation":[{"name":"Department of Hematology Azienda Ospedaliera Citt\u00e0 della Salute e della Scienza  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Di Rocco","sequence":"additional","affiliation":[{"name":"Department of Cellular Biotechnology and Hematology, Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2112-2651","authenticated-orcid":false,"given":"Pier Luigi","family":"Zinzani","sequence":"additional","affiliation":[{"name":"Institute of Hematology, University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3995-8817","authenticated-orcid":false,"given":"Francesco","family":"Zaja","sequence":"additional","affiliation":[{"name":"Clinical Ematologica, DAME University of Udine  Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Piva","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences Center for Experimental Research and Medical Studies, University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Inghirami","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences Center for Experimental Research and Medical Studies, University of Torino  Torino Italy"},{"name":"Pathology and Laboratory Medicines Weill Cornell Medical College  New York New 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