{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:20:40Z","timestamp":1747146040903,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T00:00:00Z","timestamp":1558396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["21267 Mynerva"],"award-info":[{"award-number":["21267 Mynerva"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["GR\u20102013\u201002358238","GR\u20102016\u201002362631"],"award-info":[{"award-number":["GR\u20102013\u201002358238","GR\u20102016\u201002362631"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Systemic mastocytosis (SM) is characterized by extreme heterogeneity of manifestations and prognosis. Several disease\u2010related biomarkers, including clinical, hematological and molecular variables, have been correlated with prognosis. Although relevant, the mutation profile closely reflects the WHO classification that has per se prognostic value. High\u2010risk mutations (HRM) are largely confined to advanced forms, and thus fail in providing information regarding progression and outcome in the not\u2010advanced variants. In this work, we studied hematopoietic cells by multi\u2010parameter flow cytometry (MFC) in order to highlight dysplastic traits that might provide insights into outcome. A score previously validated for myelodysplastic syndromes, with high reproducibility in standard diagnostics, was used. The application of an MFC score to a cohort of 71 SM cases, concurrently genotyped for configuring a HRM category, resulted in the identification of two separate patients' categories (MFC+ and MFC\u2010) characterized by significantly different clinical and laboratory features at presentation. The extent of dysplasia by MFC tended to parallel WHO\u2010category and genotype\u2010related stratification. MFC+ patients had shorter survival compared to MFC\u2010 ones, for whom the incidence of progression and\/or death was virtually null. Of note, MFC score remained prognostically informative in unadvanced subsets. Furthermore, the integration of MFC and HRM was an independent predictor for outcome, also overcoming WHO\u2010categories in multivariate analysis for EFS. Our results support the use of MFC analysis in the evaluation of patients with SM, alone and in combination with HRM, for refinement of prognosis assessment.<\/jats:p>","DOI":"10.1002\/ajh.25506","type":"journal-article","created":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T19:54:11Z","timestamp":1557172451000},"page":"845-852","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Myelodysplasia as assessed by multiparameter flow cytometry refines prognostic stratification provided by genotypic risk in systemic mastocytosis"],"prefix":"10.1002","volume":"94","author":[{"given":"Francesco","family":"Mannelli","sequence":"first","affiliation":[{"name":"CRIMM, Centro di Ricerca e Innovazione per le Malattie Mieloproliferative, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica, Denothe Excellence Center Universit\u00e0 degli Studi Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Gesullo","sequence":"additional","affiliation":[{"name":"CRIMM, Centro di Ricerca e Innovazione per le Malattie Mieloproliferative, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica, Denothe Excellence Center Universit\u00e0 degli Studi Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giada","family":"Rotunno","sequence":"additional","affiliation":[{"name":"CRIMM, Centro di Ricerca e Innovazione per le Malattie Mieloproliferative, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica, Denothe Excellence Center Universit\u00e0 degli Studi Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalisa","family":"Pacilli","sequence":"additional","affiliation":[{"name":"CRIMM, Centro di Ricerca e Innovazione per le Malattie Mieloproliferative, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica, Denothe Excellence Center Universit\u00e0 degli Studi Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Bencini","sequence":"additional","affiliation":[{"name":"Centro Diagnostico di Citofluorimetria e Immunoterapia, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica Denothe Excellence Center Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Annunziato","sequence":"additional","affiliation":[{"name":"Centro Diagnostico di Citofluorimetria e Immunoterapia, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica Denothe Excellence Center Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Zanotti","sequence":"additional","affiliation":[{"name":"Multidisplinary Outpatients Clinic for Mastocytosis (GISM) Azienda Ospedaliera Universitaria Integrata of Verona Verona Italy"},{"name":"Department of Medicine, Haematology Unit Azienda Ospedaliera Universitaria Integrata of Verona Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Scaffidi","sequence":"additional","affiliation":[{"name":"Multidisplinary Outpatients Clinic for Mastocytosis (GISM) Azienda Ospedaliera Universitaria Integrata of Verona Verona Italy"},{"name":"Department of Medicine, Haematology Unit Azienda Ospedaliera Universitaria Integrata of Verona Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiorina","family":"Giona","sequence":"additional","affiliation":[{"name":"Ematologia Dipartimento di Medicina Traslazionale e di Precisione, Universit\u00e0 Sapienza Roma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelina","family":"Santopietro","sequence":"additional","affiliation":[{"name":"Ematologia Dipartimento di Medicina Traslazionale e di Precisione, Universit\u00e0 Sapienza Roma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Grifoni","sequence":"additional","affiliation":[{"name":"UOC Ematologia Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico di Milano Milano Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Pieri","sequence":"additional","affiliation":[{"name":"CRIMM, Centro di Ricerca e Innovazione per le Malattie Mieloproliferative, Azienda Ospedaliera Universitaria Careggi, Dipartimento di Medicina Sperimentale e Clinica, Denothe Excellence Center Universit\u00e0 degli Studi Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1809-284X","authenticated-orcid":false,"given":"Paola","family":"Guglielmelli","sequence":"additional","affiliation":[{"name":"CRIMM, Centro di Ricerca e Innovazione per le Malattie Mieloproliferative, Azienda Ospedaliera 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