{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:26:55Z","timestamp":1783618015272,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T00:00:00Z","timestamp":1560816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bleeding is frequent among patients with thrombocytopenia. We studied whether in vitro platelet function predicts future bleeding in patients with acute myeloid leukemia (AML), and thrombocytopenia. Adult AML patients with platelet count &lt;5.0\u2009\u00d7\u200910<jats:sup>10<\/jats:sup>\/L were included. Detailed bleeding history and blood samples were collected at inclusion and every 7 days. We analyzed hematology and coagulation parameters. With flow cytometry we evaluated platelet activation (activated GPIIb\/IIIa, P\u2010selectin, and CD63 expression), and platelet aggregation. Agonists were thrombin\u2010receptor activating peptide (TRAP) and collagen\u2010related peptide (CRP\u2010XL). During 18\u2009months, sixty participants were enrolled and followed for a total of 114\u2009weeks. Bleeding occurred in 52 weeks, and was not associated with clinical, hematology or coagulation parameters. Predictors of bleeding were assessed using multivariate logistic regression, adjusted for platelet count, sex, and age. Bleeding history and receiver operating curves were compared using c\u2010index. Reduced TRAP\u2010induced platelet aggregation had Odds ratio 3.00 (95% confidence interval [CI] 1.38\u20106.60). Reduced CRP\u2010XL\u2010induced platelet aggregation had Odds ratio 4.00 (95%\u2010CI 1.70\u20109.20) for bleeding. Overall, C\u2010index was 0.71 for the models including platelet aggregation results, 0.72 for activated GPIIb\/IIIa\u2010positive platelets after stimulation with TRAP, 0.68 for percent P\u2010selectin positive platelets with TRAP and 0.63 for the platelet count. Among patients receiving no platelet transfusion, C\u2010index was 0.83\u20130.91 for bleeding; highest for models using platelet aggregation. Change in platelet aggregation did not correlate with the number of platelet concentrates administered. In conclusion, platelet aggregation and platelet activation results predict bleeding better than platelet count alone, among AML patients with thrombocytopenia.<\/jats:p>","DOI":"10.1002\/ajh.25512","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T14:39:13Z","timestamp":1558622353000},"page":"891-901","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Platelet function tests predict bleeding in patients with acute myeloid leukemia and thrombocytopenia"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2035-0169","authenticated-orcid":false,"given":"Pernille","family":"Just Vinholt","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry and Pharmacology Odense University Hospital  Odense Denmark"},{"name":"OPEN, Odense Patient data Explorative Network Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustav","family":"H\u00f8jrup Knudsen","sequence":"additional","affiliation":[{"name":"Department of Haematology Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Signe","family":"Sperling","sequence":"additional","affiliation":[{"name":"Department of Haematology Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Frederiksen","sequence":"additional","affiliation":[{"name":"Department of Haematology Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Odense University Hospital  Odense 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