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As we recently described reactivity of the B\u2010cell receptors of LP cells of some NLPHL cases with <jats:italic>Moraxella<\/jats:italic> spp. proteins, we hypothesized that LP cells could present peptides to rosetting T cells in a major histocompatibility complex class II (MHCII)\u2010bound manner. Rosetting PD1<jats:sup>+<\/jats:sup> T cells were present in the majority of NLPHL cases, both in typical (17\/20) and variant patterns (16\/19). In most cases, T\u2010cell rosettes were CD69<jats:sup>+<\/jats:sup> (typical NLPHL, 17\/20; NLPHL variant, 14\/19). Furthermore, both MHCII alpha and beta chains were expressed in the LP cells in 23\/39 NLPHL. Proximity ligation assay and confocal laser imaging demonstrated interaction of the MHCII beta chain expressed by the LP cells and the T\u2010cell receptor alpha chain expressed by rosetting T cells. We thus conclude that rosetting T cells in NLPHL express markers that are encountered after antigenic exposure, that MHCII is expressed by the LP cells, and that LP cells interact with rosetting T cells in an immunological synapse in a subset of cases. As they likely receive growth stimulatory signals in this way, blockade of this interaction, for example, by PD1\u2010directed checkpoint inhibitors, could be a treatment option in a subset of cases in the future.<\/jats:p>","DOI":"10.1002\/ajh.25972","type":"journal-article","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T07:33:24Z","timestamp":1598254404000},"page":"1495-1502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Lymphocyte predominant cells of nodular lymphocyte predominant Hodgkin lymphoma interact with rosetting T cells in an immunological synapse"],"prefix":"10.1002","volume":"95","author":[{"given":"Julia","family":"Bein","sequence":"first","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology Goethe University  Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenz","family":"Thurner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I and Jos\u00e9 Carreras Center for Immuno\u2010 and Gene Therapy Saarland University Medical School  Homburg\/Saar Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin\u2010Leo","family":"Hansmann","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology Goethe University  Frankfurt am Main Germany"},{"name":"Frankfurt Institute of Advanced Studies  Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3424-1091","authenticated-orcid":false,"given":"Sylvia","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology Goethe University  Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,3]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1889","article-title":"European Task Force on Lymphoma project on lymphocyte predominance Hodgkin disease: histologic and immunohistologic analysis of submitted cases reveals 2 types of Hodgkin disease with a nodular growth pattern and abundant lymphocytes","volume":"96","author":"Anagnostopoulos I","year":"2000","journal-title":"Blood"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.17.9337"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080809"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078812"},{"key":"e_1_2_8_6_1","first-page":"29","article-title":"Diffuse lymphocyte\u2010predominant Hodgkin's disease (diffuse paragranuloma). 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