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At least 12 p53 protein isoforms have been identified as a result of a combination of alternative splicing, alternative promoters and\/or alternative transcription site starts, which are grouped as \u03b1, \u03b2, \u03b3, from transactivation domain (TA), long, and short isoforms. Nowadays, there are no studies evaluating the expression of p53 isoforms and its clinical relevance in multiple myeloma (MM). We used capillary nanoimmunoassay to quantify the expression of p53 protein isoforms in CD138\u2010purified samples from 156 patients with newly diagnosed MM who were treated as part of the PETHEMA\/GEM2012 clinical trial and investigated their prognostic impact. Quantitative real\u2010time polymerase chain reaction was used to corroborate the results at RNA levels. Low and high levels of expression of short and TAp53\u03b2\/\u03b3 isoforms, respectively, were associated with adverse prognosis in MM patients. Multivariate Cox models identified high levels of TAp53\u03b2\/\u03b3 (hazard ratio [HR], 4.49; <jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001) and high\u2010risk cytogenetics (HR, 2.69; <jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001) as independent prognostic factors associated with shorter time to progression. The current cytogenetic\u2010risk classification was notably improved when expression levels of p53 protein isoforms were incorporated, whereby high\u2010risk MM expressing high levels of short isoforms had significantly longer survival than high\u2010risk patients with low levels of these isoforms. This is the first study that demonstrates the prognostic value of p53 isoforms in MM patients, providing new insights on the role of p53 protein dysregulation in MM biology.<\/jats:p>","DOI":"10.1002\/ajh.26507","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T14:06:50Z","timestamp":1645452410000},"page":"700-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Expression of p53 protein isoforms predicts survival in patients with multiple myeloma"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2541-7254","authenticated-orcid":false,"given":"Elizabeta A.","family":"Rojas","sequence":"first","affiliation":[{"name":"Hematology Department University Hospital of Salamanca, IBSAL  Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC)  Salamanca 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