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OGM can dramatically simplify lab workflow by reducing multiple tests (conventional karyotype, fluorescence in situ hybridization [FISH], and chromosomal microarray) into one test. The superior ability to detect structural variation across the genome removes the need for reflex FISH studies, which can dramatically reduce cost and turnaround time per sample. Parallel studies of OGM versus standard of care testing have demonstrated it can detect and resolve more abnormalities than karyotyping or FISH. However, like many molecular tests that normalize copy number it can have difficulty with non\u2010diploid karyotypes. This Test of the Month review will summarize how the technique works, review the strengths and weaknesses of OGM compared to standard of care techniques and illustrate how the technique is likely to change front line testing in many hematologic malignancies\u2014including summarizing the clinical utility in acute myeloid leukemia, myelodysplastic syndromes, and B cell acute lymphoblastic leukemia.<\/jats:p>","DOI":"10.1002\/ajh.26587","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T03:59:32Z","timestamp":1652414372000},"page":"975-982","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Optical genome mapping for structural variation analysis in hematologic malignancies"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9927-4914","authenticated-orcid":false,"given":"Adam C.","family":"Smith","sequence":"first","affiliation":[{"name":"Laboratory Medicine Program University Health Network  Toronto Ontario Canada"},{"name":"The Department of Laboratory Medicine and Pathobiology, Faculty of Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelia","family":"Neveling","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7829-5249","authenticated-orcid":false,"given":"Rashmi","family":"Kanagal\u2010Shamanna","sequence":"additional","affiliation":[{"name":"Department of Hematopathology and Molecular Diagnostics The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/243290a0"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"94","article-title":"Exclusion of chromosomal mosaicism: tables of 90%, 95% and 99% confidence limits and comments on use","volume":"29","author":"Hook EB","year":"1977","journal-title":"Am J Hum Genet"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201008148\u2010z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0268\u2010960X(03)00040\u20107"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201003\u2010643544.The"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201008\u2010733196"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2021.0002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","unstructured":"Xu J. 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