{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:09:22Z","timestamp":1780452562425,"version":"3.54.1"},"reference-count":92,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007316","name":"Division of Cancer Prevention, National Cancer Institute","doi-asserted-by":"publisher","award":["1 F30CA278500\u201001A1"],"award-info":[{"award-number":["1 F30CA278500\u201001A1"]}],"id":[{"id":"10.13039\/100007316","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Acute myeloid leukemia (AML) is a genetically heterogeneous disease with high rates of relapse after initial treatment. Identifying measurable residual disease (MRD) following initial therapy is essential to assess response, predict patient outcomes, and identify those in need of additional intervention. Currently, MRD analysis relies on invasive, serial bone marrow (BM) biopsies, which complicate sample availability and processing time and negatively impact patient experience. Additionally, finding a positive result can generate more questions than answers, causing anxiety for both the patient and the provider. Peripheral blood (PB) evaluation has shown promise in detecting MRD and is now recommended by the European Leukemia Net for AML for certain genetic abnormalities. PB\u2010based sampling allows for more frequent testing intervals and better temporal resolution of malignant expansion while sparing patients additional invasive procedures. In this review, we will discuss the current state of PB testing for MRD evaluation with a focus on next\u2010generation sequencing methodologies that are capable of MRD detection across AML subtypes.<\/jats:p>","DOI":"10.1002\/ajh.27586","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T06:39:22Z","timestamp":1736231962000},"page":"638-651","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Breaking the Bone Marrow Barrier: Peripheral Blood as a Gateway to Measurable Residual Disease Detection in Acute Myelogenous Leukemia"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9945-9791","authenticated-orcid":false,"given":"John T.","family":"Butler","sequence":"first","affiliation":[{"name":"Radiation Medicine and Applied Science, Moores Cancer Center University of California San Diego  La Jolla California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Yashar","sequence":"additional","affiliation":[{"name":"Knight Cancer Institute Oregon Health &amp; Science University  Portland Oregon USA"},{"name":"Division of Oncologic Sciences, Department of Medicine Oregon Health &amp; Science University  Portland Oregon USA"},{"name":"Department of Biomedical Engineering Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan","family":"Swords","sequence":"additional","affiliation":[{"name":"Division of Oncologic Sciences, Department of Medicine Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"e_1_2_19_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08851"},{"key":"e_1_2_19_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61727-9"},{"key":"e_1_2_19_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-604496"},{"key":"e_1_2_19_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01613-1"},{"key":"e_1_2_19_6_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201847546"},{"key":"e_1_2_19_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164390"},{"key":"e_1_2_19_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2023-180812"},{"key":"e_1_2_19_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-020-00765-5"},{"key":"e_1_2_19_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1015"},{"key":"e_1_2_19_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0623-z"},{"key":"e_1_2_19_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021013626"},{"key":"e_1_2_19_13_1","unstructured":"\u201cSEER \u201d 2024."},{"key":"e_1_2_19_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.45.9628"},{"key":"e_1_2_19_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-363291"},{"key":"e_1_2_19_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-506725"},{"key":"e_1_2_19_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054790"},{"key":"e_1_2_19_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-023-01424-6"},{"key":"e_1_2_19_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-024-02275-x"},{"key":"e_1_2_19_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2020.4600"},{"key":"e_1_2_19_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-09-801498"},{"key":"e_1_2_19_22_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2022.282034"},{"key":"e_1_2_19_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403405"},{"key":"e_1_2_19_24_1","volume-title":"Leukemia","author":"Li W.","year":"2022"},{"key":"e_1_2_19_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.113"},{"key":"e_1_2_19_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-577593"},{"key":"e_1_2_19_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019004226"},{"key":"e_1_2_19_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.30"},{"key":"e_1_2_19_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020007626"},{"key":"e_1_2_19_30_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.1363"},{"key":"e_1_2_19_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2022.106822"},{"key":"e_1_2_19_32_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2023.283520"},{"key":"e_1_2_19_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-02-829911"},{"key":"e_1_2_19_34_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1507471"},{"key":"e_1_2_19_35_1","doi-asserted-by":"publisher","DOI":"10.4081\/hr.2020.8957"},{"key":"e_1_2_19_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-76933-2"},{"key":"e_1_2_19_37_1","unstructured":"ThermoFisher \u201cOncomine Myeloid MRD NGS Assay [Website] \u201d 2023 https:\/\/www.oncomine.com\/myeloid\u2010mrd."},{"key":"e_1_2_19_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-020-00484-5"},{"key":"e_1_2_19_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2021.07.010"},{"key":"e_1_2_19_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2023-189525"},{"key":"e_1_2_19_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-99-115071"},{"key":"e_1_2_19_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-024-02337-0"},{"key":"e_1_2_19_43_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10432"},{"key":"e_1_2_19_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.255"},{"key":"e_1_2_19_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020006219"},{"key":"e_1_2_19_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22444-1"},{"key":"e_1_2_19_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-022-00270-y"},{"key":"e_1_2_19_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019001156"},{"key":"e_1_2_19_49_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-745273"},{"key":"e_1_2_19_50_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.67.1875"},{"key":"e_1_2_19_51_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-461749"},{"key":"e_1_2_19_52_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2023010416"},{"key":"e_1_2_19_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.07.002"},{"key":"e_1_2_19_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-01175-9"},{"key":"e_1_2_19_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-11339-w"},{"key":"e_1_2_19_56_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.22.01114"},{"key":"e_1_2_19_57_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2017.102"},{"key":"e_1_2_19_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.158"},{"key":"e_1_2_19_59_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.183335"},{"key":"e_1_2_19_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11295"},{"key":"e_1_2_19_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00090"},{"key":"e_1_2_19_62_1","volume-title":"Extracellular Vesicle Trafficking Enforces Microenvironmental Dysregulation in Acute Myeloid Leukemia","author":"Butler J. T.","year":"2020"},{"key":"e_1_2_19_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-319"},{"key":"e_1_2_19_64_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2016.1174578"},{"key":"e_1_2_19_65_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v5.29289"},{"key":"e_1_2_19_66_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-2184"},{"key":"e_1_2_19_67_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021004621"},{"key":"e_1_2_19_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2018.03.007"},{"key":"e_1_2_19_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00133"},{"key":"e_1_2_19_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2010.11.015"},{"key":"e_1_2_19_71_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-1681"},{"key":"e_1_2_19_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-021-01316-z"},{"key":"e_1_2_19_73_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25429"},{"key":"e_1_2_19_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105013"},{"key":"e_1_2_19_75_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-022-02707-w"},{"key":"e_1_2_19_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09982-5"},{"key":"e_1_2_19_77_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.21856"},{"key":"e_1_2_19_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.01.031"},{"key":"e_1_2_19_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.07.017"},{"key":"e_1_2_19_80_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0233-1"},{"key":"e_1_2_19_81_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019001181"},{"key":"e_1_2_19_82_1","doi-asserted-by":"publisher","DOI":"10.1158\/2643-3230.BCD-21-0050"},{"key":"e_1_2_19_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2864-x"},{"key":"e_1_2_19_84_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-020-00791-w"},{"key":"e_1_2_19_85_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00249"},{"key":"e_1_2_19_86_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022007378"},{"key":"e_1_2_19_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/gim.2014.122"},{"key":"e_1_2_19_88_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3642"},{"key":"e_1_2_19_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0915-3"},{"key":"e_1_2_19_90_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12213"},{"key":"e_1_2_19_91_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-024-02493-4"},{"key":"e_1_2_19_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2020.07.014"},{"key":"e_1_2_19_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0393-0"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.27586","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T05:05:50Z","timestamp":1752901550000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.27586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,7]]},"references-count":92,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/ajh.27586"],"URL":"https:\/\/doi.org\/10.1002\/ajh.27586","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,7]]},"assertion":[{"value":"2024-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}