{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:09:02Z","timestamp":1782187742475,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The optimal conditioning schedule and CD34+ cell dose for autologous stem cell transplantation (ASCT) for AL amyloidosis is unknown. Patients (<jats:italic>n<\/jats:italic>\u2009=\u20091704) who underwent ASCT for AL amyloidosis between 2003 and 2020 in 9 centers were included. Data on melphalan conditioning dose, number of conditioning days, whether a rest day between conditioning and stem cell infusion was given or not, and infused CD34<jats:sup>+<\/jats:sup> cell dose were collected. Full\u2010dose melphalan (\u2265\u2009180\u2009mg\/m<jats:sup>2<\/jats:sup>) was administered in 63.7% of the patients, and 80.4% had melphalan split into 2\u2010day conditioning. A rest day (day \u22121) between the conditioning regimen and stem cell infusion was provided in 52.5% of patients. The median infused CD34<jats:sup>+<\/jats:sup> cell count was 4.7\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/kg. An infused CD34<jats:sup>+<\/jats:sup> cell count \u2265\u20094.5\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/kg was associated with a shorter time to neutrophil and platelet engraftment. In a nominal regression analysis, full\u2010dose melphalan, 1\u2010day melphalan conditioning, and omitting a rest day between conditioning and stem cell infusion were independent predictors of post\u2010ASCT higher deep hematological response. The median follow\u2010up was 8.6\u2009years, and 38% of patients died. Independent predictors of superior overall survival in multivariate Cox regression analysis included full\u2010dose melphalan, having no rest day, and infused CD34<jats:sup>+<\/jats:sup> cells \u2265\u20094.5\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/kg. Independent predictors of higher day\u2010100 transplant\u2010related mortality (TRM) in nominal logistic regression analysis included poorer performance status, NT\u2010proBNP\/BNP \u2265\u20091800\/400\u2009pg\/mL, serum albumin &lt;\u20092.5\u2009g\/dL, CD34<jats:sup>+<\/jats:sup> cells &lt;\u20094.5\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/kg, and not having a rest day. In conclusion, 1 day of melphalan conditioning and administration of CD34<jats:sup>+<\/jats:sup> cells \u2265\u20094.5\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/kg are recommended for ASCT in AL amyloidosis.<\/jats:p>","DOI":"10.1002\/ajh.27685","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T08:27:07Z","timestamp":1744360027000},"page":"1141-1151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Impact of Melphalan Conditioning and <scp>CD34<\/scp><sup>+<\/sup> Cell Dose and Schedule on Post\u2010Transplant Outcomes in <scp>AL<\/scp> Amyloidosis"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2210-2174","authenticated-orcid":false,"given":"Eli","family":"Muchtar","sequence":"first","affiliation":[{"name":"Division of Hematology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6307-2445","authenticated-orcid":false,"given":"Vaishali","family":"Sanchorawala","sequence":"additional","affiliation":[{"name":"Section of Hematology and Oncology, Amyloidosis Center Boston University Chobanian &amp; Avedisian School of Medicine, Boston Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1775-2703","authenticated-orcid":false,"given":"Hamza","family":"Hassan","sequence":"additional","affiliation":[{"name":"Section of Hematology and Oncology, Amyloidosis Center Boston University Chobanian &amp; Avedisian School of Medicine, Boston Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1917-6746","authenticated-orcid":false,"given":"Ute","family":"Hegenbart","sequence":"additional","affiliation":[{"name":"Medical Department V, Amyloidosis Center University of Heidelberg  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Sch\u00f6nland","sequence":"additional","affiliation":[{"name":"Medical Department V, Amyloidosis Center University of Heidelberg  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8545-3292","authenticated-orcid":false,"given":"Hans C.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Lymphoma and Myeloma, Division of Cancer Medicine University of Texas M.D. Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2876-2886","authenticated-orcid":false,"given":"Muzaffar","family":"Qazilbash","sequence":"additional","affiliation":[{"name":"Department of Lymphoma and Myeloma, Division of Cancer Medicine University of Texas M.D. Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Kin","sequence":"additional","affiliation":[{"name":"Barbara Ann Karmanos Cancer Institute  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Zonder","sequence":"additional","affiliation":[{"name":"Barbara Ann Karmanos Cancer Institute  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eapen","family":"Jacob","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Buadi","sequence":"additional","affiliation":[{"name":"Division of Hematology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8780-9512","authenticated-orcid":false,"given":"Angela","family":"Dispenzieri","sequence":"additional","affiliation":[{"name":"Division of Hematology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Dingli","sequence":"additional","affiliation":[{"name":"Division of Hematology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally","family":"Arai","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Chin","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine  Stanford California USA"},{"name":"University of Central Florida College of Medicine  Orlando Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7336-3003","authenticated-orcid":false,"given":"Rajshekhar","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology Columbia University Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Lentzsch","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology Columbia University Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3990-394X","authenticated-orcid":false,"given":"Hila","family":"Magen","sequence":"additional","affiliation":[{"name":"Hematology Institute, Chaim Sheba Medical Center, Tel Hashomer, and Faculty of Medicine Tel Aviv University  Tel\u2010Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eden","family":"Shkury","sequence":"additional","affiliation":[{"name":"Hematology Institute, Chaim Sheba Medical Center, Tel Hashomer, and Faculty of Medicine Tel Aviv University  Tel\u2010Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin","family":"Sarubbi","sequence":"additional","affiliation":[{"name":"Division of Hematologic Oncology Memorial Sloan Kettering Cancer Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Landau","sequence":"additional","affiliation":[{"name":"Division of Hematologic Oncology Memorial Sloan Kettering Cancer Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3853-5196","authenticated-orcid":false,"given":"Morie","family":"Gertz","sequence":"additional","affiliation":[{"name":"Division of Hematology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703200"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/13506129.2021.2002841"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2028631"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-020-00397-w"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-330738"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2018.06.018"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2017.68"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26641"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2021.03.012"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17980"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704826"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-145-4-200608150-00004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.37.7614"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-02-767475"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-02-767467"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.62.4015"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-020-0916-3"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2014.56"},{"issue":"3","key":"e_1_2_9_20_1","first-page":"472","article-title":"A Modern Primer on Light Chain Amyloidosis in 592 Patients With Mass Spectrometry\u2010Verified Typing","volume":"94","author":"Muchtar E.","year":"2019","journal-title":"Mayo Clinic Proceedings"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0034-3"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2013.53"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2018.04.028"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-021-01093-z"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2013.152"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-017-0056-6"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12759"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488806775269179"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1995.1051"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26237305"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2012.01795.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-024-01165-w"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705763"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02202.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtct.2021.11.004"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.21278"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-11-3503"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-208355"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2009.331"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.8.2726"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402909"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38498257378.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2017.07.015"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.27685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T10:14:14Z","timestamp":1754907254000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.27685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,11]]},"references-count":42,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/ajh.27685"],"URL":"https:\/\/doi.org\/10.1002\/ajh.27685","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,11]]},"assertion":[{"value":"2025-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}