{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:56:13Z","timestamp":1782939373843,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008637","name":"Center for Strategic Scientific Initiatives, National Cancer Institute","doi-asserted-by":"publisher","award":["1RO1CA233945\u201001"],"award-info":[{"award-number":["1RO1CA233945\u201001"]}],"id":[{"id":"10.13039\/100008637","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Nodular lymphocyte\u2010predominant Hodgkin Lymphoma (NLPHL) may be an antigen\u2010driven malignancy. Recent studies demonstrated that in NLPHL patients, lymphoma B\u2010cell receptor can bind proteins derived from <jats:italic>\n<jats:styled-content style=\"fixed-case\">Moraxella catarrhalis<\/jats:styled-content> (MC) and <jats:styled-content style=\"fixed-case\">Rothia mucilaginosa<\/jats:styled-content> (RM).<\/jats:italic> We examined whether <jats:italic>MC<\/jats:italic> and <jats:italic>RM<\/jats:italic> can be detected in NLPHL lymph nodes. The presence of <jats:italic>MC<\/jats:italic> DNA and RNA was tested by polymerase chain reaction (PCR) and RNAScope in\u00a0situ hybridization (ISH), respectively. The presence of <jats:italic>RM<\/jats:italic> DNA was tested by PCR. We demonstrated the presence of <jats:italic>MC<\/jats:italic> DNA in 29 of 49 (59.2%) NLPHL patient tumor samples and RNA in 14 of 39 biopsies (35.9%), with good concordance between the assays, considering lower RNA stability in older biopsies and lower sensitivity of ISH compared to PCR. In comparison, 36 lymph nodes with follicular hyperplasia and a specimen with tubular adenoma were negative for <jats:italic>MC<\/jats:italic> by PCR, and only 6 of 37 non\u2010NLPHL lymphoma tissues (16.2%) were positive for detection of <jats:italic>MC<\/jats:italic> by PCR, demonstrating a statistically higher detection rate of <jats:italic>MC<\/jats:italic> in patients with NLPHL (<jats:italic>p<\/jats:italic>\u2009=\u20090.00006). <jats:italic>MC<\/jats:italic> was detected in NLPHL samples at diagnosis and relapses, and there was no statistical difference in the detection rate for <jats:italic>MC<\/jats:italic> between IgD positive and negative samples. In contrast, <jats:italic>RM<\/jats:italic> was detected by PCR in 9 of 49 (18.4%) NLPHL patient tumor samples, always concomitantly with <jats:italic>MC<\/jats:italic> without statistical difference in comparison to other lymphomas. The high prevalence of <jats:italic>MC<\/jats:italic> in NLPHL tumor tissues provides direct evidence for its association with NLPHL.<\/jats:p>","DOI":"10.1002\/ajh.27762","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T04:52:41Z","timestamp":1751604761000},"page":"1543-1556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Association Between <scp><i>Moraxella catarrhalis<\/i><\/scp> and Nodular Lymphocyte\u2010Predominant Hodgkin Lymphoma"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-9013","authenticated-orcid":false,"given":"Izidore S.","family":"Lossos","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Medicine University of Miami  Miami Florida USA"},{"name":"Department of Molecular and Cellular Pharmacology University of Miami  Miami Florida USA"},{"name":"Sylvester Comprehensive Cancer Center  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna","family":"Patel","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5353-7950","authenticated-orcid":false,"given":"Kamilia N.","family":"Moalem","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Laser","sequence":"additional","affiliation":[{"name":"Bio\u2010Techne  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo Y.","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pathology  Duarte California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anamarija M.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Chapman","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center  Miami Florida USA"},{"name":"Division of Hematopathology, Department of Pathology University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-06-2508"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09198-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-341560"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1492"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-325217"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019004044"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2016.05.009"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5567"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.17.9337"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01620-2"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1979.tb03011.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015851"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000107"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16375-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2023.282698"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00314-2"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/521194"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199411000-00009"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200412-1747OC"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01270-14"},{"issue":"49","key":"e_1_2_9_22_1","first-page":"2763","article-title":"DNA Extraction From Paraffin Embedded Material for Genetic and Epigenetic Analyses","author":"Pikor L. 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