{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T22:24:00Z","timestamp":1759703040834},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":11149,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1976,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two new variants of glucose 6\u2010phosphate dehydrogenase (G6PD) deficiency associated with chronic nonspherocytic hemolytic anemia were discovered in Japan. Gd(\u2010) Tokushima was found in a 17\u2010year\u2010old male whose erythrocytes contained 4.4% of normal enzyme activity. Partially purified enzyme revealed a main band of normal electrophoretic mobility with additional two minor bands of different mobility; normal Km G6P, and Km NADP five\u2010 to sixfold higher than normal; normal utilization of 2\u2010deoxy\u2010G6P, galactose\u20106P, and deamino\u2010NADP; marked thermal instability; a normal pH curve; and normal Ki NADPH. The hemolytic anemia was moderate to severe. Gd(\u2010) Tokyo was characterized from a 15\u2010year\u2010old male who had chronic nonspherocytic hemolytic anemia of mild degree. The erythrocytes contained 3% of normal enzyme activity, and partially purified enzyme revealed slow electrophoretic mobility (90% of normal for both a tris\u2010hydrochloride buffer system and a tris\u2010EDTA\u2010borate buffer system, and 70% of normal for a phosphate buffer system); normal Km G6P and Km NADP; normal utilization of 2\u2010deoxy\u2010G6P, galactose\u20106P, and deamino\u2010NADP; greatly increased thermal instability; a normal pH curve; and normal Ki NADPH. These two variants are clearly different from hitherto described G6PD variants, including the Japanese variants Gd(\u2010) Heian and Gd(\u2010) Kyoto. The mothers of both Gd(\u2010) Tokushima and Gd(\u2010) Tokyo were found to be heterozygote by an ascorbate\u2010cyanide test.<\/jats:p>","DOI":"10.1002\/ajh.2830010408","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T04:38:48Z","timestamp":1172032728000},"page":"433-442","source":"Crossref","is-referenced-by-count":15,"title":["Two new glucose 6\u2010phosphate dehydrogenase variants associated with congenital nonspherocytic hemolytic anemia found in Japan: Gd(\u2010) tokushima and gd(\u2010) tokyo"],"prefix":"10.1002","volume":"1","author":[{"given":"Shiro","family":"Miwa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichiro","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigenobu","family":"Abe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kageoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Shinohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Isobe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisomu","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"243","article-title":"Human glucose\u20106\u2010phosphate dehydrogenase variants","volume":"45","author":"Yoshida A","year":"1971","journal-title":"Bull WHO"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1973.tb01823.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1973.tb01749.x"},{"key":"e_1_2_1_5_2","first-page":"587","article-title":"An\u00e9mie h\u00e9molytique cong\u00e9nitale nonsph\u00e9rocytdire par d\u00e9ficit en glucose\u20106\u2010phosphate\u2010dehydrog\u00e9nase \u00e9rythrocytaire","volume":"14","author":"Kahn A","year":"1974","journal-title":"Nouv Rev Franc H\u00e9mat"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1182\/blood.V44.3.323.323","article-title":"A new variant of glucose\u20106\u2010phosphate dehydrogenase deficiency hereditary hemolytic anemia, G6PD Cornell: erythrocyte, leukocyte, and platelet studies","volume":"44","author":"Miller DR","year":"1974","journal-title":"Blood"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000208300"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1159\/000459413","article-title":"G6PD Baudelocque: a new unstable variant characterized in cultured fibroblasts","volume":"18","author":"Junien C","year":"1974","journal-title":"Enzyme"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1974.tb00810.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.75.263"},{"key":"e_1_2_1_11_2","first-page":"585","article-title":"Glucose\u20106\u2010phosphate dehydrogenase deficiency. 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