{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:18:42Z","timestamp":1756991922994},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":11149,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1976,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hyperimmune antisera to chromatographically purified hemoglobins F and A<jats:sub>2<\/jats:sub>, were produced in rabbits and made specific for the immunogen by adsorption with normal human hemoglobin A conjugated to cyanogen bromide\u2010activated agarose. A radioimmunoassay was established that permitted identification and quantitation of each of these two minor hemoglobins in hemolysates containing other hemoglobin components. The quantities of hemoglobins A<jats:sub>2<\/jats:sub>, and\/or F present in hemolysates of individuals with \u03b2\u2010thalassemia, sickle cell anemia, Hb\u2010C disease, and other hematological disorders were determined immunochemically, and the results were compared to values obtained by microcolumn chromatography for the measurement of Hb\u2010A<jats:sub>2<\/jats:sub> or with the alkali denaturation technique in quantitating Hb\u2010F. The immunoassay procedure has a greater sensitivity than other commonly employed techniques and can detect as little as 0.05 \u03bcg of these hemoglobins.<\/jats:p>","DOI":"10.1002\/ajh.2830010411","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T04:38:48Z","timestamp":1172032728000},"page":"459-469","source":"Crossref","is-referenced-by-count":31,"title":["Specific radioimmunochemical identification and quantitation of hemoglobins a2 and f"],"prefix":"10.1002","volume":"1","author":[{"given":"Fred A.","family":"Garver","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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