{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T13:04:28Z","timestamp":1765112668087},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":10783,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1977,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>), at concentrations \u2265 5 \u00d7 10<jats:sup>4<\/jats:sup> ng\/ml, induced discocyte \u2192 echinocyte transformation of saline\u2010suspended hemoglobin (Hb) AA and SS erythrocytes. This erythrocyte transformation is concentration\u2010dependent and is reversible at room temperature after 90\u2013120 min. The Hb SS erythrocytes treated with PGE<jats:sub>2<\/jats:sub> did not exhibit accelerated sickling or increased formation of sickled echinocvtes. Erythrocytes suspended in autologous plasma treated with PGE<jats:sub>2<\/jats:sub>, 2 \u00d7 10<jats:sup>6<\/jats:sup> ng\/ml, did not exhibit echinocytic transformation probably because of drug binding to the plasma proteins. Other in vitro studies showed that PGE<jats:sub>2<\/jats:sub> of concentrations of 10\u2013500 ng\/ml had no adverse effects on intact, plasma\u2010suspended Hb SS erythrocytes. These Hb SS erythrocytes were examined for changes in morphology, potassium and calcium flux, and blood viscosity under oxygenated and hypoxic conditions.<\/jats:p>","DOI":"10.1002\/ajh.2830020205","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T04:29:29Z","timestamp":1172032169000},"page":"133-143","source":"Crossref","is-referenced-by-count":3,"title":["Prostaglandin e<sub>2<\/sub>\u2010hemoglobin AA and SS erythrocyte interaction (prostaglandin\u2010erythrocyte interaction)"],"prefix":"10.1002","volume":"2","author":[{"given":"Joseph T.","family":"Sobota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn A.","family":"Gruber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry J.","family":"Gilbertson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerome","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.174.4008.512"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1093\/clinchem\/19.1.23","article-title":"Detection of prostaglandin induction of erythrocyte sickling","volume":"19","author":"Johnson M","year":"1973","journal-title":"Clin Chem"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(75)90060-X"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4021.539"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(73)90011-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197311082891920"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(73)90137-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197204062861419"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1016\/S0021-9258(19)41604-9","article-title":"Stoichiometry of compounds bound to human erythrocytes in relation to morphology","volume":"250","author":"Lovrien R","year":"1975","journal-title":"J Biol Chem"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88062-9_1"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197207132870201"},{"key":"e_1_2_1_13_2","first-page":"68","volume-title":"The Analysis of Variance","author":"Scheff\u00e9 H","year":"1959"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1001\/archinte.1974.00320160071007","article-title":"Rheological aspects of sickle cell disease","volume":"133","author":"Klug PP","year":"1974","journal-title":"Arch Intern Med"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.133.1.86"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.39.1.31"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/246105a0"},{"key":"e_1_2_1_18_2","unstructured":"EatonJW BergerE WhiteJGandJacobHS:Effects of calcium on Hb SS erythrocytes: Evidence that calcium accumulation underlies formation of irreversibly sickled red cells. In: Proc Symposium on the Molecular and Cellular Aspects of Sickle Cell Disease Dallas Texas 1975(in press)."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(74)90062-5"},{"key":"e_1_2_1_20_2","first-page":"121","article-title":"Scanning electron microscopy of erythrocyte deformation: The influence of a calcium ionophore, A23187","volume":"13","author":"White JG","year":"1976","journal-title":"Seminars Hemat"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(68)90078-3"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88062-9_8"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(72)90140-3"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"6713","DOI":"10.1016\/S0021-9258(19)45905-X","article-title":"On the metabolism of prostaglandins E1 and E2 in man","volume":"246","author":"Hamburg M","year":"1971","journal-title":"J Biol Chem"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830020205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830020205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T03:17:11Z","timestamp":1699845431000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830020205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,1]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1977,1]]}},"alternative-id":["10.1002\/ajh.2830020205"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830020205","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,1]]}}}